活性红M-3BE脱色真菌的筛选及脱色性能研究

Screening of decolorizing fungi and decolorizing research for active red M-3BE

从自然环境中分离到2株对染料活性红M-3BE具有明显脱色效果的真菌，经形态学和26SrDNA序列分析，将其鉴定为Fusarium oxysporum和Geosmithia viridis。采用初始浓度50mg／LM-3BE的液体培养基同步脱色培养，F．oxysporum在24h内对M-3BE的脱色率为96％，G．viridis在36h内的脱色率为82％。对脱色酶系的检测结果表明，脱色过程中F．oxysporum能产生LiP和MnP，G．viridis则仅产生LiP。此外，F．oxysporu和G．viridis对另外7种染料的脱色率也可分别达到16％～100％和83．3％～100％。

2 strains of fungi, with high decolorizing ability of active red M-3BE were isolated from the environment. According to the morphological characteristics and sequence analysis of 26S rDNA, the strains were identified as Fusarium oxysporum and Geosmithia viridis. The decolorization rate of 50 mg/L M-3BE by F. oxysporum could reach 96% within 24 h, and that of G. viridis＇s was 82% within 36 h. Detection results of decolorizing enzyme system indicated that F. oxysporum could produce two enzymes, Lip and MnP, however G. viridis only one enzyme, LiP. Extensive decolorization by F. oxysporu （ 16% - 100% ） and G. viridis （83. 3% 100% ） was obtained with other seven dyes.