腹水巨噬细胞分离及在抗瘤活性测定中的应用

Isolating Macrophages from Ascites in patient and Determining Macrophages Antitumor Cytotoxic Activity

目的:建立临床实用的巨噬细胞(MΦ)分离技术。方法:腹腔注入植物血球凝集素(PHA),收集肝癌患者腹水,并经离心沉淀、贴壁培养等处理。结果:纯度>85%,活细胞>99%;用 LPS 激活后 MΦ杀瘤细胞毒活性37.4%,对照组为0.8%(P<0.005)。结论:从腹水中分离 MΦ取材容易,操作简便,不需特殊设备,纯化效果好,可满足 MΦ体外培养及杀瘤活性测定等研究应用,具有较高的实用价值。

Objective:The tuna of this experiment was to set up a practical method of isolating macrophages for use in clinical study.Methods:The peritoneal macrophages were obtained by collecting ascites in patient with hepatocellular carcinoma,centrifugual sedimentation,and anchornge-dependant method.Before experiment,the patients were injected PHA into abdominal cavity,to increase the number and purity of macrophages.Resu/ts:The purity of macrophages was mote than 85% and the living macrophages more than 99%.Antitumor cytotoxic activity of activated macrophages by LPS was 37.4% and non-activated macrophages 0.8%(P<0.005).Conclusion:The method of isolating macrophages from ascites was simple and practical.