中药清热方及IL-18抗体抗急性免疫性肝坏死动物模型的实验性研究

The Research of Acute Immunized Hepatonecrosis Animal Model Treated with the Traditional Chinese Medicine and Anti-IL-18

目的通过急性免疫性肝坏死动物模型的建立并予中药清热方及IL-18抗体干预,了解中药清热方及IL-18抗体在急性免疫性肝坏死动物模型形成中的作用及其机制。方法取BALB/c小鼠24只,随机分为四组:A组(正常对照组)6只,其他18只采用卡介苗+内毒素诱导法建立急性免疫性肝损伤模型并随机分为B组、C组、D组。B组为单纯病理组,C组、D组两组在模型形成前分别予中药清热方及IL-18抗体干预。实验结束取所有BALB/c小鼠肝组织予病理学炎症活动度计分,血清IL-18采用酶联免疫吸附法检测,肝组织IL-18、TNF-α、IFN-γ采用逆转录-聚合酶链式反应技术检测。结果肝组织炎症活动度计分(HAI):C组、D组分别为5.76±1.43,3.33±1.63,与B组(19.00±0.55)比较,差异有统计学意义(P<0.05);D组与C组比较,差异有统计学意义(P<0.05)。血清IL-18:B、C、D组明显较A组增高,差异有统计学意义(P<0.05);各组间比较,差异也有统计学意义(P<0.05)。各组肝组织IL-18、INF-γ、TNF-αmRNA表达水平的比较,B组、C组、D组明显较A组增高,差异有统计学意义(P<0.05);各组间比较,差异也有统计学意义(P<0.05)。结论IL-18、INF-γ、TNF-α在急性免疫性肝坏死动物模型的形成中发挥了重要作用;中药清热方及IL-18抗体可以干预急性免疫性肝坏死动物模型的形成。

Objective Through founded acute immunized hepatonecrosis animal model and treating with the traditional Chinese medicine and anti-IL-18 to understand the mechanism of acute immunized hepatonecrosis. Method Twenty-four BALB/c mouse were selected and divided into four groups randomly：group A（Ga）,groupB（Gb）, group C（Gc） and group D（Gd）, in Gb, Gc and Gd groups used BCG＋ LPS were used to develop acute immunized hepatonecrosis animal model. Gc and Cad were treated with the traditional Chinese medicine and anti-IL-18 respective. After experiment the hepatic tissue of HAIwere detected, the IL-18,TNF-α,IFN-γ were chected by RT-PCR. Results The HAl of mouse in Gc and Gd were 5.76 ± 1.43 and 3.33 ± 1.63 respective, there was significant difference （P〈0.05） ;serum IL-18 in Gb,Gc,Gd was higher than that in Ga（P〈0.05） ;moreover, there was significant difference in Gb, Gc, Gd （P 〈 0.05） ; the IL-18, INF-γ, TNF-α mRNA of hepatic tissue were higher than that in Ga（P〈0.05）;moreover, there was significant difference in Gb,Gc,Gd （P 〈 0.05）. Conclusion The IL-18, INF-γ and TNF-α play an important pole in acute immunized hepatonecrosis animal model, the traditional Chinese medicine and anti-IL-18 can step in the formation of acute immunized hepatonecrosis.