卵黄抗体对幽门螺杆菌感染沙土鼠的预防试验

Inhibitory Effect of Egg-yolk Antibody on Experimental Helicobacter Pylori Infection in Mongolian Gerbils

目的在成功研制出抗幽门螺杆菌(Hp)卵黄抗体的基础上,利用蒙古沙土鼠作为实验动物,观察抗Hp卵黄抗体对Hp感染的防治作用。方法将144只实验鼠随机分为4组,每组36只。Ⅰ、Ⅱ、Ⅲ组分别灌服生理盐水、复合抗生素和抗Hp卵黄抗体,1次/d,连续13 d;Ⅳ组皮下注射抗Hp卵黄抗体,1次/d,48 h后再注射1次。在首次用药后48 h口服接种幽门螺杆菌(ATCC43504)2.75×108CFU(布氏培养液)。结果在接种后第15、30、45天,Ⅰ组鼠胃内均有大量Hp定植,感染率为100%;Ⅱ、Ⅲ、Ⅳ组的感染率均<23%,3组之间的差异无统计学意义(P<0.05)。结论灌服和注射抗Hp卵黄抗体,可以抑制沙土鼠感染Hp,其抑制效果与抗生素相仿。

Objective Inhibitory effect of egg-yolk antibody on experimental Helicobacter pylori （Hp） infection in Mongolian gerbil was observed as soon as egg-yolk antibody against Hp was prepared. Method The experiment gerbils were divided into 4 groups（36 mice/group） and then normal saline was taken orally normal saline in group Ⅰ , combination of antibiotics in Group Ⅱ, egg-yolk antibody against Hp in Group Ⅲ for 13 days （one time/d）. The egg-yolk antibody was injected hypodermically twice with a 48 h interval in group Ⅳ. 48 h after first time administration the mice were inoculated with 2.75×10^8 colony formation units（CFUs） of Hp（ATCC43504） bacteria grown in Brucella broth. Results There were more Hp in the stomach on the 7th,15th,30th,45th day after inoculation. The infection rate was 100% in group Ⅰ , but low than 23% in Group Ⅱ, Group Ⅲ or group Ⅳ. Conclusion The egg-yolk antibody against Hp swallowed or injected might decrease HP infection in the stomach of Mongolian gerbils, and there is no obvious difference in effect between the egg-yolk antibody and the antibiotic（P〈0.05）.