龙胆科生药中2种苦甙的含量测定

Determination of Two Main Bitter Secoiridoid Glycosides in Gentianaceae

报道龙胆科植物５属３２种５９个产地的生药，加甲醇超声后，直接用ＨＰＬＣ法测定了獐牙菜苦甙（ｓｗｅｒｔｉａｍａｒｉｎ）和龙胆苦甙（ｇｅｎｔｉｏｐｉｃｒｏｓｉｄｅ）的含量．流动相：１０％甲醇→５０％甲醇梯度洗脱，柱温：５０℃，内标：３－（邻－甲氧基－苯氧基）１，２－丙二醇（ｇｕａｉｆｅｎｅｓｉｎ），检测波长：２５４ｎｍ，流速：１ｍＬ／ｍｉｎ，进样５μＬ．获得满意结果．

This paper reports the quantitative determination of swertiamarin and gentiopicroside by HPLC method.Shimadzu Model LC-6A liquid chromatographic system equipped with ODS column(TSK gel 80 Ts,5μm,150mm×4 6mm stainless steel)was used for this experiment.The HPLC conditions were as follows:mobile phase:10% methanol→50% methanol(40min.gradient),column temperature:50℃,internal standard:guaifenesin,detection wavelength:254nm,flow rate:1mL/min and injection volume:5μL.. The contents of swertiamarin and gentiopicroside in 32 species of Gentianaceae were thus determined.The quantitative evaluation of “Long Dan”,“Qin Jiu”,“Dang Yao”and “Qing Ye Dan”were discussed according to the results of determination.