兔膝关节腔注射^(32)P-磷酸铬胶体后外周血淋巴细胞染色体畸变率的变化

The Change of Chromosome Aberration Rate for Peripheral Blood Lymphocytes After Injection of Colloidal Chromic Phosphate ~32P into Rabbit Knee Joint Cavities

目的观察32P-磷酸铬胶体注入兔类风湿关节炎模型膝关节腔后对外周血淋巴细胞染色体畸变率的影响。方法9只新西兰兔随机分成3组,A组3只为正常对照;B组3只为模型对照;C组3只诱导成模型1周后,右膝关节腔内各注射32P-胶体磷酸铬44.4MBq。3组均在C组核素注射后2个月时经耳缘静脉采血,其中C组另在核素注射前、注射后3d时采血。经培养后比较各组分裂中期淋巴细胞染色体畸变率的变化。结果A、B、C3组外周血淋巴细胞染色体观察均未见双着丝粒,无着丝粒断片率3组无明显差异(P>0.05)。C组各时间点外周血淋巴细胞染色体观察也未发现双着丝粒,无着丝粒断片率也无明显差异(P>0.05)。结论关节腔注射实验剂量的32P-磷酸铬胶体,兔外周血淋巴细胞畸变率的波动在正常范围内,说明放射性滑膜切除术是一种安全的治疗方法。

Objective To study the impact on the chromosome aberration rate for peripheral blood lymphocytes after injecting colloidal chromic phosphate ^32p into knee joint cavities of rabbit models of rheumatoid arthritis. Methods Nine rabbits were divided into three groups randomly. Three rabbits in group A were for normal comparison and three rabbits in group B for model comparison. One week after the three rabbits in group C have been induced as models, 44.4 MBq colloidal chromic phosphate ^32p was injected into the right knee joint cavity. In all of the three groups blood samples were taken from the ear-rim veins upon two months after the nuclein injection in group C. For group C, a blood sampling three days before and after the nuclein injection was conducted. After cultivation, examination and comparison of the changes in lymphocytes chromosome aberration rate were conducted during the interim division in different groups. Results No obvious twin-centromere in the lymphocytes chromosome of peripheral blood was observed in all three groups. No distinct differences was observed （ P 〉 0.05） in comparison of fragment rates. No twin-centromere was discovered in lymphocytes chromosome in peripheral blood, and no obvious difference （ P 〉 0.05） in fragment rates at all scheduled time in group C. Conclusion After injecting colloidal chromic phosphate ^32p in lab test dosages into articular cavities, the fluctuation of lymph cell chromosome aberration rate in peripheral blood of the rabbit is within the normal range, which proves that radioisotope synovectomy is a safe treatment method.