内侧前脑束不同部位注射6-羟多巴胺建立帕金森病大鼠模型的对比性研究

Study on Comparing Different Means of Injection 6-HODA into Medial Fore Brain Bundles and Establish the Rat Model with Parkinson Disease

目的建立简单、易行、成功率高的制作帕金森病大鼠模型的方法。方法注射同等剂量的6-羟多巴胺于内侧前脑束不同部位(第1组注射位点:位点1:前囟后1.8mm,右侧2.0mm,背腹8.0mm;位点2:前囟后1.8mm,右侧3.0mm,背腹7.5mm。第2组注射位点:位点1:前囟后1.8mm,右侧2.5mm,背腹8.0mm;位点2:前囟后1.8mm,右侧2.5mm,背腹7.5mm),造成黑质多巴胺能神经元的损坏,运用阿朴吗啡诱发旋转试验及免疫组化检验模型是否成功。比较两组注射位点的成功率。结果采用第1组注射位点的成功率为51%,第2组注射位点的成功率为77%,显著高于第1组(P<0.01)。结论采用第2组注射位点制作帕金森病大鼠模型成功率高、易行。

Objective To establish a simple and effective rat model of Parkinson disease（PD） . Methods In two groups, 6-HODA was injected into medial forebrain bundles at the two coordinates on the right sides at the same dosage. In first group, two burr holes were made with a dental drill, two coordinates are 2. 0 mm lateral to the midline, 8.0 mm in vertically, and - 1.8 mm anterior-posterior at the zero point to the bregma; 3.0 mm lateral to the midline, 7. 5 mm in vertically, and - 1.8 mm anterior-posterior at the zero point to the bregma. In second group, one burr hole was made, two coordinates are 2. 5 mm lateral to the midline, 8.0 mm and 7. 5 mm in vertically, and - 1.8 mm anterior-posterior at the zero point to the bregma. After 6 weeks, apomorphine-induced rotation test and immunohisto- chemiscal test were performed to examine the disease progress in this rat model. Results In group 1,51% of them were proved to be successful by apomorphine induction. In group 2, the successful ratio is 77%. There was significant difference（P 〈 0.01） between the two groups. Conclusion This rat model in PD can be created in high successful ratio by two injection points with 6-HODA, which are 2. 5 mm lateral to the midline, 8.0 mm and 7. 5 mm in vertically, and - 1.8 mm anterior-posterior at the zero point to the bregma.