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采用cDNA芯片检测Krüppel样因子4过表达对C_2C_(12)细胞基因表达谱的影响 被引量：1

Transcriptional Profiling of C_2C_(12) Cells Overexpressed Krüppel-Like Factor
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摘要 目的采用cDNA芯片检测Krüppel样因子4过表达对C2C12细胞中基因表达谱的影响。方法用cDNA芯片检测Krüppel样因子4过表达的C2C12细胞中基因表达谱的改变(以空载体细胞为对照);用MEDLINE、Genomatix等生物信息学数据库和分析软件对在Krüppel样因子4过表达的C2C12细胞中表达发生改变的已命名基因进行功能和细胞信号通路分析。结果采用cDNA芯片筛选到在Krüppel样因子4过表达的C2C12细胞中表达发生改变的基因605个;其中下调的基因为250个,已命名基因为155个;上调的基因为355个,已命名基因为255个;其中包含多个炎症相关基因和凋亡相关基因。上述炎症相关基因属于13条细胞信号通路,凋亡相关基因属于9条细胞信号通路。结论Krüppel样因子4过表达能够引起C2C12细胞中多个下游基因表达发生改变。 Aim To detect the change of transcriptional profiling of C2C12 cells overexpressed krüppel-like factor 4 （KLF4）. Methods eDNA chip containing 14000 mouse cDNAs were employed to investigate the expression of genes influenced by KLF4 overexpression in C2C12 cells; bioinformatics database and software such as MEDLINE and Genomatix were performed to analyze the above genes. Results Two hundred and fifity genes were down-regnlated in C2C12 cells overexpressed KLF4, with 155 known; Whereas 355 genes were up-restated, with 255 known. Several genes about inflammatory or apoptosis were included. Conclusion Many downstream genes were influenced by KLF4 ovcrexpression in C2C12 cells.
作者 易宇欣 刘瑛 刘俊文 张华莉 周新民 肖献忠
机构地区 中南大学湘雅医学院病理生理学教研室 湘雅二医院心胸外科
出处 《中国动脉硬化杂志》 CAS CSCD 2007年第8期585-590,共6页 Chinese Journal of Arteriosclerosis
基金 国家自然科学基金(30400169 30571746 30571846 30330280) 国家973重点资助项目(G2000056908)
关键词 病理学与病理生理学 CDNA芯片 Krüppel样因子4 C2C12细胞 生物信息学 基因转染 信号通路 cDNA Chip Krüppel-Like Factor 4 C2C12 Cells Bioinformatics Gene Transfection Signal Transduction Pathway
分类号 R363 [医药卫生—病理学]
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参考文献20

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共引文献3

同被引文献10

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中国动脉硬化杂志
2007年 第8期

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