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血管紧张素Ⅱ对血管平滑肌细胞钙化的影响及信号通路 被引量：1

Effects of Angiotensin Ⅱ on the Calcification in Vascular Smooth Muscle Cells and Its Signal Pathway
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摘要 目的在钙化大鼠主动脉血管平滑肌细胞上观察血管紧张素Ⅱ对钙化的影响及其信号通道。方法用β磷酸甘油制备钙化的大鼠血管平滑肌细胞,再以血管紧张素Ⅱ,血管紧张素Ⅱ1型受体阻断剂缬沙坦,选择性蛋白激酶A或蛋白激酶C抑制剂等干预,通过Von Kossa染色及检测钙含量、碱性磷酸酶活性、骨钙素浓度和核心结合因子a1 mRNA表达来探讨血管紧张素Ⅱ对钙化的影响及其信号通道。结果血管紧张素Ⅱ增加钙化大鼠血管平滑肌细胞的钙含量、碱性磷酸酶活性、骨钙素浓度和核心结合因子a1 mRNA表达(P<0.05);而血管紧张素Ⅱ1型受体阻断剂和选择性蛋白激酶C抑制剂可阻断血管紧张素Ⅱ对血管平滑肌细胞的钙含量、碱性磷酸酶活性、骨钙素浓度和核心结合因子a1 mRNA表达的影响(P<0.05)。结论血管紧张素Ⅱ可促进β磷酸甘油诱导的血管平滑肌细胞钙化,其途径是通过血管紧张素Ⅱ1型受体,胞内信号途径是蛋白激酶C核心结合因子a1途径。 Aim To clarify the effects of angiotensin Ⅱ on the calcification in rat vascular smooth muscle cells （ VSMCs ） and its signal transduction. Methods Calcification of cultured rat VSMCs was prepared by incubation with β-glycerophosphate. Calcification was confirmed by Von Kossa staining, and calcium content, alkaline phosphatases activity, osteocalcin and core binding factor alpha 1 （ Cbfal ） mRNA expression were measured. Results Angiotensin Ⅱ enhanced the increase of calcium content, alkaline phosphatases activity, osteocalcin concentration and Cbfal mRNA expression in calcified VSMCs （ P 〈 0.05）. Angiotensin Ⅱ type 1 receptor blocker valsartan and selective protein kinase C （PKC） inhibitor inhibited the effects of angiotensin Ⅱ on vascular calcification（ P 〈 0.05）. Condusions These data suggested that β-glycerophosphate-induced calcification in VSMCs was enhanced by angiotensin Ⅱ through angiotensin Ⅱ type 1 receptor and PKC-Cbfal pathway.
作者 何文 马虹 张焰 彭龙云
机构地区 中山大学附属第一医院心内科
出处 《中国动脉硬化杂志》 CAS CSCD 2007年第8期609-612,共4页 Chinese Journal of Arteriosclerosis
关键词 内科学 心血管 血管紧张素Ⅱ 大鼠 血管平滑肌细胞 钙化 信号通道 Cardiovascular Angiotensin Ⅱ Rat Vascular Smooth Muscle Cell Calcification Signal Pathway
分类号 R5 [医药卫生—内科学]
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参考文献10

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共引文献23

同被引文献40

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中国动脉硬化杂志
2007年 第8期

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