三七皂甙对急性坏死性胰腺炎大鼠胰腺NF-κB活性及胰腺损伤的影响

The Effect of Panax Notoginseng Saponins on Activation of NF-κB in ANP Rats

目的探讨三七总皂甙能否抑制急性坏死性胰腺炎大鼠胰腺NF-κB的活性,减轻ANP时胰腺病理损害。方法SD大鼠30只。随机分为3组每组10只:假手术模型组(SO组),急性坏死性胰腺炎组(ANP组),三七总皂甙预处理(PNS组),SO组和ANP组造模前1h腹腔注射0.9%生理盐水(0.1ml/100g),PNS组造模前1h腹腔注射50mg/ml三七总皂甙(0.1ml/100g)。5%牛磺胆酸钠大鼠胆胰管逆行注射制备大鼠急性坏死性胰腺炎模型,SO组进腹后仅翻动胰腺和十二指肠后关腹,不注入牛磺胆酸钠。各组造模后6h点处死大鼠,取胰腺组织测定湿/干重比和测其中NF-κB活性。结果ANP组造模后6h胰腺组织NF-κB阳性细胞数明显高于SO组(P<0.01)。结论NF-κB在大鼠急性坏死性胰腺炎胰腺组织中明显激活;三七总皂甙可以在体内抑制NF-κB在胰腺组织中的激活,减轻各脏器的病理损害,可望用于治疗ANP。

Objective To demonstrates：Does panax notoginseng saponins, the inhibitor of NF-κB, decrease the function and pathological injury in the ANP rat. Methods Thirty Sprague-Dawley rats were randomly divided into three groups： Sham operation group（ group SO） , acute necrotizing pancreatitis group （group ANP） , panax notoginseng saponins preconditioning group （group PNS）. There were 10 rats in each group. Rat acute necrotizing panereatitis model was induced by retrograde injection of 5% sodium taurocholate to pancreatic duct （0.1 ml/100 g）. The rats in the panax notoginseng saponins preconditioning group were given by intraperitonel injection of 0.1 ml/100g panax notoginseng saponins（50 mg/ml） one hour before model establishment, and those in the other two groups were given 0.9% physiological saline. Put rat to death at 6 hour after the model were made, Pancreatic were made to detected the activity of NF-KB by immunohistochemistry. Results NF-KB aetivity of pancreatitis： The nurmber of positive cells of pulmonary in SNP group emarkably increased compared to SO group ＇after model establishment at 6 hour（P 〈 0.01 ）. Conclusions NF-κB is activated obviously in lung in rat acute necrotizing pancreatitis; Inhibiting of NF-κB by panax notoginseng saponins, and pathological damage of pancreas were also reduced. Those show that ANP might he treated by panax notoginseng saponins in the future.