肝病大鼠创伤弧菌攻击后肺损伤和血清细胞因子的检测

Lung injury and changes of serum cytokines in hepatopathy rats attacked by Vibrio vulnificus

目的:探讨创伤弧菌(Vibrio vulnificus,Vv)所致肝病大鼠肺损伤、血清细胞因子变化及其相关性。方法:用四氯化碳制备慢性肝病大鼠18只,分Vv攻击和肝病大鼠对照组,制备Vv致死性感染多器官损伤肝病大鼠模型(5.0×107cfu/只),在观察肺细胞超微结构改变的同时检测大鼠血清TNF-α、IL-1β和IL-10的含量变化,与接受相同菌量Vv攻击正常大鼠,正常大鼠、肝病大鼠阴性对照组(注入生理盐水,n=9/组)相比较。结果:在接种Vv攻击4小时后肝病大鼠组仅存活1只,5小时后全部死亡(0/9,0%)。正常大鼠Vv攻击组5小时存活8只(8/9,88.9%)。肝病大鼠组存活率较Vv攻击正常大鼠组显著降低(P<0.01);血清细胞因子的含量检测提示Vv攻击后肝病大鼠血清IL-10(32.5pg/ml)显著低于正常大鼠Vv攻击组(71.8pg/ml,P<0.05),而TNF-α(54.3pg/ml)、IL-1β(421.1pg/ml)则分别明显高于正常大鼠Vv攻击组(40.7和255.4pg/ml),差异有显著性(P<0.01)。肝病大鼠的肺II型上皮细胞严重空泡样改变,而正常大鼠以同样Vv菌量攻击后未见相应改变。结论:结果提示肝病大鼠的死亡率、血清IL-1β、TNF-α水平和肺组织的损伤程度均显著高于接受相同菌量的非肝病大鼠,而IL-10则相反,结果可为肝病病人感染Vv后快速进入多器官功能障碍综合征(MODS)、高死亡率提供实验依据。

Objective：To study lung injury and changes of serum cytokines in hepatopathy rats attacked by Vibrio vulnificus （Vv）. Methods：Eighteen chronic hepatopathy rats prepared by carbon tetrachloride were divided into Vv attack group（5.0 × 107 cfu each） and control group（treated with normal saline ）. The changes of uhrastructure of lung were observed and the concentration of serum TNF-α, IL-1β, and IL-10 were also detected. Results：There was significant different between the 5 h survival rate of hepatopathy group and normal group after attacked by the same amount of Vv （0/9 vs 8/9,P 〈0. 01 ）. serum IL-10 level of hepatopathy ratswas significantly lower than that of normal rats （32. 5pg/ml vs 71.8pg/ml, P 〈0. 05）, however, TNF-α and IL-1 levels of hepatopathy rats were significantly higher than those of normal rats （54. 3pg/ml vs 40. 7pg/ml, P 〈 0. 01 ; 421. l pg/ml vs 255.4pg/ml, P 〈 0. 01 ） o The observation of ultrastructure of lung showed that mitochondrial crystal decreased, swoUen and vacuole changes occurred, indicating that lung were severely damaged. However, not observed in normal rats group after Vv attack. Conclusions damage in the lung acted as an important feature of the fatality of V. vulnificus infections and a positive relationship with the amount of TNF-α and IL-1β