Ku70 mRNA表达与人非小细胞肺癌化学治疗耐受性关系的研究

The affection of Ku70 mRNA expression to tumor and drug resistance in human lung cancer

目的:分析Ku70 mRNA在人正常肺组织、未经化疗和多次化疗的非小细胞肺癌组织的定量表达水平与化学治疗耐受性的关系。方法:采用RT-PCR方法对26例正常肺组织和56例肺癌组织中的Ku70 mRNA水平进行半定量测定,分析Ku70 mRNA表达水平与化学治疗耐受性关系。结果:肺癌组织中Ku70 mRNA表达水平明显高于正常肺组织(P<0.01);经多次化疗的肺癌组织中Ku70 mRNA的表达水平明显高于未经化疗的肺癌组织(P<0.01)。结论:随化疗次数增加,Ku70 mRNA在肺癌组织中表达量的明显增高可能与人肺癌组织对化学治疗耐受性相关。

Objective：To investigate the relationship between Ku70 mRNA expression in non-neoplasm, in normal appearing pulmonary tissues, and in lung cancer tissue of pre-or post-chemotherapy and drug resistance. Methods： Ku70 mRNA in the lung tissues was measured by reverse transcription polymerase chain reaction （RT-PCR）. The samples were extracted form 26 non-neoplasm, norreal appearing pulmonary tissues and 56 lung cancer tissues. Results： Non-neoplasm, normal appearing pulmonary tissue group expressed Ku70 mRNA lower than lung cancer tissue group（P 〈0. 01 ）. And the lung cancer tissue with multiple chemotherapy （N≥2） expressed Ku70 mRNA more than the lung cancer tissue without chemotherapy （P 〈 0. 01 ）. Conclusion：Ku70 mRNA expression may concern with chemotherapy resistance in human lung cancer, which is up-regulated following chemotherapy.