熟地黄中活性成分甘露三糖提取工艺研究

Study on Extraction Technology of Manninotriose in Radix Rehmanniae Praeparata

目的:筛选熟地黄中活性成分甘露三糖的最佳提取条件。方法:以高效液相蒸发光散射(HPLC-ELSD)为含量测定方法,采用正交实验,以浸膏得率和甘露三糖含量为考察指标,对甘露三糖的提取工艺条件进行优选。结果:所考察的因素中,对甘露三糖的提取工艺影响程度为:提取次数>浸提时间>加水量。结论:提取甘露三糖的最佳条件为:沸水浸提时间为6h,加4倍水,提取2次,具有工艺简单、成本低、收率稳定等优点。

Objective： To optimize water extraction technology of Orthogonal design and HPLC-ELSD were used in the determination manninotriose in Radix Rehmanniae Praeparata. Methods： and assessment of the extractive yield and the content of manninotriose. Results： The importance of the factors affecting water extraction decreased in the following order： extraction frequency, extraction time, the amount of water. Conclusion： The optimum water extraction condition was as follows： four times of water, extracting two times, six hours per time. The process can be conducted simply, conveniently and inexpensively.