摘要
Hemoglobin was used as a mimetic peroxidase in the catalytic chemiluminescence reaction of lumino and hydrogen peroxide. The hemoglobin-catalyzed reaction was coupled with the oxidation reaction of cholesterol catalyzed by COA(cholesterol oxydase , COA.) to determine total cholesterol(TC)and free cholesterol (FC) in human serum by flow injection chemiluminescence method. The sampling frequency was 30 s/h. The linear range for free cholesterol is 5.6×10-7mol/L～3.8×10-5mol/L and the detection limit is 3.0×10-7mol/L. The linear range for total cholesterol is 1.1×10-6mol/L～4.3×10-5mol/L and the detection limit is 1.5×10-7mol/L.
Hemoglobin was used as a mimetic peroxidase in the catalytic chemiluminescence reaction of lumino and hydrogen peroxide. The hemoglobin - catalyzed reaction was coupled with the oxidation reaction of cholesterol catalyzed by COA （ cholesterol oxydase , COA. ） to determine total cholesterol（TC） and free cholesterol （FC） in human serum by flow injection chemiluminescence method. The sampling frequency was 30 s/h. The linear range for free cholesterol is 5. 6 × 10^-7 mol/L -3. 8 × 10^-5mol/L and the detection limit is 3.0 × 10^-7 mol/L. The linear range for total cholesterol is 1.1 × 10^-6 mol/L - 4.3 × 10^-5 mol/L and the detection limit is 1.5 × 10^-7 mol/L.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2007年第7期791-794,共4页
Chemical Research and Application
关键词
流动注射
血红蛋白
化学发光
游离胆固醇
总胆固醇
flow injection
hemoglobin
chemiluminescence
free cholesterol
total cholesterol
