提高猪基因导入效率的研究

Study on Increasing Transgenic Efficiency in Pigs

采用显微注射的方法，将POMT／PGH、PSV40／PGH、POMT／PGH－MicrosatelliteDNA和PMHR等四种外源基因导入2592枚湖北白猪的受精卵，均获得了相应的转基因猪，基因导入的总效率为2．1％。对影响导入效率的几个因素进行了系统的实验。通过超数排卵，可以使供体母猪的获印数提高1．1倍；在异体移植的情况下，每头受体以移入20～29枚注射基因的胚胎为宜；采用自体移植，在移入胚数基本相同的情况下，其受孕率和产仔率比异体移植分别提高30．7％（P＜0．05）和15．1％（P＜0．01）。不仅提出了对转基因猪整合和表达检测的证据，而且提出了由目的基因决定的表型性状和传代方面的证据。

Four kinds of gene (such as POMT/PGH, PSV40/ PGH, POMT/PGH-Microsatellite DNA and PMHR) are microinjected into 2592 embryos of Hubei white pig, and the transgenic pigs have been obtained appropriately. Toeal transgenic efficiency is 2.1%. Some factors of effecting transgene have been tested for the system. By superovulation, the ovulation number is increased by 2.1 times. For every receptor,the implanted transgenic embryo number is 20-29. In planting the same number embryos, the pregnancy rate and pigging rate of pig with self-implant are higher than 30. 7% (p<0. 05) and 15.1% (P <0.01) approprately. The experiment brought the evidence about checking integration and expression of transgenic pig and phenotypic performances and genetic forms to be decided by aim gene.