人羟甲基戊二酸单酰辅酶A(HMG-CoA)还原酶催化域在大肠杆菌中的表达及纯化

Expression and Purification of the Catalytic Domain of Human HMG-CoA Reductase in E. coli

参照1993年Frimpong等表达仓鼠同源酶催化域的研究结果，将PCR法制备的人HMG－CoA还原酶催化域cDNA克隆入表达载体pET30，使表达产物C端融合载体编码的His－Tag，以利产物的稳定和完整产物的纯化。

In respect that the expression of Syrian Hamster HMG-CoA reductase with C-terminal EEF extension produce homogeneous protein with high catalytic activity reported by Frimpong et al in 1993, human HMG-CoA reductase cDNA prepared by PCR is inserted into expression vector PET30, so that the product will carry C-terminal fusion His-Tag for production of homogeneous protein and purification of intact product.