期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

原癌基因c-raf在精原干细胞中的表达及作用

The Expression and Effect of Proto-oncogene c-raf in Spermatogonial Stem Cells
下载PDF
导出
摘要 本研究用基因测序和BLAST检验扩增的精原干细胞DNA和c-raf的同源性;用MTT法观察c-rafASODNs(反义c-raf寡脱氧核甘酸)拮抗c-raf对体外培养精原干细胞培养的作用,探讨原癌基因c-raf对体外精原干细胞培养增殖的影响.实验结果显示扩增的精原干细胞DNA和c-raf的同源性98%;c-rafASODNs对体外培养的精原干细胞存活呈剂量依赖性抑制作用(P<0.05).提示c-raf在精原干细胞中表达;原癌基因c-raf促进精原干细胞体外培养的增殖. DNA sequencing and BLAST were used to identify the homology between the amplified spermatogonial stem cell＇s DNA and c-raf. The effect of antisense oligodeoxynucleotides（ASODNs） on proliferation of spermatogonial stem cells in vitro was observed by MTT assay. The results showed that the homology between the amplified spermatogonial stem cell＇s DNA and the c-raf was 98 % and c-raf ASODNs decreased the survival rate of spermatogonial stem cells in a does-dependent manner （P 〈 0.05）. It＇s suggested that c-raf was not only expressed in spermatogonial stem cell but also enhanced the survival and proliferation of spermatogonial stem cell.
作者 闫登科 徐斯凡 王晶磊 陈家详
机构地区 南昌大学医学院 中央民族大学生命与环境科学学院中国少数民族传统医学研究中心
出处 《中央民族大学学报（自然科学版）》 2007年第3期233-237,共5页 Journal of Minzu University of China(Natural Sciences Edition)
基金 国家自然科学基金项目(No.30360032) 江西省科技重点攻关项目(No.2004B0300500) 江西省教育厅科技资助项目
关键词 精原干细胞 C-RAF 反义寡脱氧核甘酸 spermatogonial stem cell c- raf antisense oligodeoxynucleotides
分类号 R321.1 [医药卫生—人体解剖和组织胚胎学]
  • 相关文献

参考文献13

  • 1杨俊玲,徐斯凡.原癌基因在生精细胞中的表达及其作用[J].中华男科学杂志,2005,11(7):532-535. 被引量：3
  • 2[2]HAGEMANN C,RAPP U R.Isotype-Specific Functions of Raf Kinases[J].Experimental Cell Research,1999,253(1):34-46.
  • 3[3]KOLBUS A,PILAT S,HUSAK Z,et al.Raf-1 antagonizes erythroid differentiation by restraining caspase activation[J].J Exp Med.,2002,196(10):1347-1353.
  • 4[4]MIKULA M,SCHREIBER M,HUSAK Z,et al.Embryonic lethality and fetal liver apoptosis in mice lacking the c-raf-1 gene[J].EMBO,2001,20(8):1952-1962.
  • 5[5]ALGECIRAS-SCHIMNICH A,BARNHART B C,PETER M E.Apoptosis-independent functions of killer caspases[J].Curr Opin Cell Biol.,2002,14(6):721-726.
  • 6[6]HWANG Y H,CHOI J Y,KIM S,et al.Over-expression of c-raf-1 proto-oncogene in liver cirrhosis and hepatocellular carcinoma[J].Hepatol Res.,2004,29(2):113-121.
  • 7[7]LAU Q C,BRUSSELBASH S,UULLER R.Abrogation of c-Raf expression induces apoptosis in tumor cells[J].Oncogene,1998,16(14):1899-1902.
  • 8[8]O'DWYER P J,STEVENSON J P,GALLAQHERM,et al.c-raf-1 Depletion and Tumor Responses in Patients Treated with thecraf-1 Antisense Oligodeoxynucleotide ISIS 5132(CGP 69846A)[J].Clinical Cancer Research,1999,5(12):3977-3982.
  • 9[9]CIOFFL C L,GARAY M,JOHNSTON J F,et al.Selective Inhibition of A-Raf and C-Raf mRNA Expression by antisense Oligodeoxynucleotides in Rat Vascular Smooth Muscle Cells:Role of A-Raf and C-Raf in Serum-Induced proliferation[J].Molicular pharmacology,1997,51(3):383-389.
  • 10杨俊玲,徐斯凡,陈加祥,余荣娇,曾辛,王晶磊,况海斌.大鼠A型精原细胞的体外培养[J].解剖学报,2006,37(4):468-472. 被引量：8

二级参考文献35

  • 1Boado R J，J Pharm Sci，1998年，87卷，1308页
  • 2Schrans-Stassen BH, van de Kant HJ, de Rooij DG, et al. Differential expression of c-kit in mouse undifferentiated and differentiating type A spermatogonia [ J ]. Endocrinology, 1999, 140(12) :5894-5900.
  • 3Kissel H, Timokhina I, Hardy MP, et al. Point mutation in kit receptor tyrosine kinase reveals essential roles for kit signaling in spermatogenesis and oogenesis without affecting other kit responses[J]. EMBO J, 2000, 19 (6) :1312-1326.
  • 4Feng LX, Ravindranath N, Dym M. Stem cell factor/c-kit up-regulates cyclin D3 and promotes cell cycle progression via the phosphoinositide 3-kinase/p70 S6 kinase pathway in spermatogonia[J]. J Biol Chem, 2000, 275 (33) :25572-25576.
  • 5Dolci S, Pellegrini M, Agostino S,et al. Signaling through extracellular signal-regulated kinase is required for spermatogonial proliferative response to stem cell factor [ J ]. J Biol Chem, 2001,276 (43) :40225-40233.
  • 6Pellegrini M, Grimaldi P, Rossi P, et al. Developmental expression of BMP4/ALK3/SMAD5 signaling pathway in the mouse testis: a potential role of BMP4 in spermatogonia differentiation [ J ].J Cell Sci, 2003, 116 (Pt 16) :3363-3372.
  • 7Tsurnta JK, Eddy EM, OBrien DA. Insulin-like growth factor-Ⅱ/cation-independent mannose 6-phosphate receptor mediates paracrine interactions during spermatogonial development[ J]. Biol Reprod, 2000, 63 (4): 1006-1013.
  • 8Cobellis G, Meccariello R, Fienga G, et al. Cytoplasmic and nuclear Fos protein forms regulate resumption of spermatogenesis in the frog, Rana esculenta[ J]. Endocrinology, 2002, 143 ( 1 ):163-170.
  • 9Chieffi P, Picascia A, Stanzione R, et al. C-Jun phosphorylation (Ser-63) in the testis of the lizard, Podarcis s. sicula[ J]. J Endocrinol, 1999 ,163 (2):337-344.
  • 10Ferrara D, Palmiero C, Branno M, et al. Testicular activity of Mos in the frog, Rana esculenta:a new role in spermatogonial proliferation[ J ]. Biol Reprod, 2004, 70 (6): 1782-1789.

共引文献19

中央民族大学学报（自然科学版）
2007年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部