胃癌组织中caspase 9的表达及其多态性变化

Expression of the caspase 9 gene and its polymorphism distribution in gastric cancer

目的:探讨胃癌中caspase 9基因的表达及其单核苷酸多态位点的分布.方法:应用Western-blot方法检测70例胃癌患者及对应癌旁正常组织中caspase 9的表达情况.应用限制性片段长度多态性技术结合测序,分析70例胃患者及100例正常人caspase 9基因rs1052576位点基因型.应用列联表法统计分析患者组和对照组SNP位点基因型及等位基因频率.结果:51.4%(36/70)的胃癌组织caspase 9基因表达明显下调,与癌旁正常组织相比差异有统计学意义(P<0.05).位于caspase 9基因第5外显子的SNP位点rs1052576的基因型频率和等位基因频率在两组人群中的分布差异有统计学意义.患者组G等位基因频率明显高于对照组(22.9% vs 13.0%,P<0.05),AG基因型频率在有淋巴结转移的患者中明显高于无淋巴结转移的患者(P<0.05).结论:caspase 9基因在胃癌中低表达,rs1052576多态位点G等位基因和胃癌的发生相关,AG基因型和淋巴结转移有关.

AIM： To explore the expression of caspase 9 in gastric cancer, and single nucleotide polymorphism （SNP） distribution of caspase 9 in gastric cancer patients and healthy individuals. METHODS： Western blotting was used to analyze the expression of caspase 9 in 70 gastric cancer patients and normal gastric tissues. We investigated the distribution of a caspase 9 gene SNP. We analyzed the genotype rs1052576 in 70 patients and 100 healthy individuals by polymerase chain reaction-restriction fragment length polymorphism. Statistical analysis was applied to analyze SNP genotype frequency and allele frequency in patients and the control group. RESULTS： In comparison with normal gastric tissues, caspase 9 gene expression was obviously down-regulated in 51,4% （36/70） of gastric cancer tissues, rs1052576 was located in exon 5 ofthe caspase 9 gene showed a significant difference between the two groups, and the G allele frequency in gastric cancer patients was higher than that in healthy controls （22.9% vs 13.0%, P 〈 0.05）. AG genotype frequency in patients with lymph node metastasis was higher than that in patients with no lymph node metastasis （P 〈 0.05）. CONCLUSION： The caspase 9 gene is associated with gastric cancer oncogenesis, rs1052576 located in exon 5 of the caspase 9 gene is associated with gastric cancer. AG genotype is associated with lymph node metastasis.