脂多糖经线粒体途径诱导细胞滋养细胞凋亡

Lipopolysaccharide induces Apoptosis of Cytotrophoblasts via Mitochondria Pathway

目的:探讨脂多糖(Lipopolysaccharide,LPS)诱导细胞滋养细胞凋亡的机制。方法:从正常早孕绒毛分离细胞滋养细胞,用无血清培养基培养。在培养基中加入不同浓度的LPS,使其终浓度分别为0、25、50、100、200ng/ml。用光镜和透射电镜观察细胞滋养细胞的形态学变化;用Western blot检测Bax、Bcl-2和Caspase-8的表达。结果:LPS作用后24h,光镜和电镜下见细胞滋养细胞出现凋亡的形态学变化;Western blot检测结果表明,LPS抑制Bcl-2表达,促进Bax和Caspase-8表达。结论:LPS能够经线粒体途径诱导细胞滋养细胞凋亡。

Objective：To investigate whether Lipopolysaccharide（LPS） can induce apoptosis in cytotrophoblasts via mitochondria pathway. Methods：Villous were collected from normal early pregnant abortions. Then cytotrophoblasts were isolated and cultured with serum-free media. Subsequently, cytotrophoblasts were treated with LPS at the concentrations of 0 （control） ,25,50,100 and 200ng/ml. Apoptosis in cytotrophoblast was tested by light microscope, and transmission electron microscope. The expressions of Bcl-2, Bax, and Caspase-3 were detected by Western blot. Results：Under light and transmission electron microscope, characteristic pathological changes of apoptosis in cytotrophoblast were observed after LPS treatment. Western blot results showed that LPS inhibited expression of Bcl-2 and promoted expression of Bax and Caspase-8. Conclusion：LPS induces apoptosis in cytotrophoblasts via mitochondria pathway.