Atopobium vaginae与细菌性阴道病关系的研究

Study on the association of Atopobium vaginae with bacterial vaginosis

目的:应用非培养方法研究Atopobium vaginae与细菌性阴道病的关系。方法:根据Nugent评分标准从15例健康妇女及17例BV患者的其阴道分泌物中提取细菌总DNA。分别选择健康妇女及BV患者样本各3例,构建16S rRNA基因克隆文库并进行测序分析。应用Atopobium vaginae16S rRNA基因的特异性引物对32例样本进行PCR扩增。结果:Atopobium vaginae是BV患者阴道菌群中的优势菌种,它存在于大部分(14/17)BV患者阴道中,健康妇女阴道中(1/15)则很罕见。结论:Atopobium vaginae与细菌性阴道病的发生有一定的相关性。

Objective：To explore the association of Atopobium vaginae with bacterial vaginosis by culture-independent method. Methods：Total microbial community DNA of 32 sampies ,including 15 healthy women and 17 patients with BV based on the Nugent criteria,was ex- tracted from vaginal swabs. The 16S rRNA gene clone libraries of 6 samples from 3 BV patients and 3 healthy women were structed and sequences analysis were done. With the specific primer for 16S rRNA gene of Atopobium vaginae,32 samples were PCR amplificated. Results：Atopobium vaginae predominated in the vaginal microflora of BV-patients. It was present in vaginal microflora of the most BVpatients（ 14 /17）, but very rarely in that of healthy women（ 1 /15）. ium vaginae is associated with the occurrence of BV.