急性冠状动脉综合征患者血清热休克蛋白60和肿瘤坏死因子-α检测

Detection of serum heat shock protein 60 and tumor necrosis factor-α in patients with acute coronary syndrome

目的:检测急性冠状动脉综合征(ACS)患者外周血热休克蛋白60(HSP60)和肿瘤坏死因子-α(TNF-α)水平,并探讨2者的相关性。方法:选择稳定型心绞痛(SAP)患者36例,ACS患者23例,以健康成人20例作为对照,采用ELISA方法检测血清HSP60和TNF-α水平。结果:ACS组血清HSP60水平高于正常对照组和SAP组(P均<0.001),SAP组和对照组间差异无统计学意义(P>0.05)。TNF-α水平在各组之间差异均无统计学意义(P均>0.05)。血清HSP60与TNF-α水平无相关性(r=-0.146,P>0.05)。结论:ACS患者血清HSP60水平升高,可反映患者体内的免疫状态。

Aim ： To investigate the levels of serum heat shock protein 60 （HSP60） and tumor necrosis factor-α（ TNF- α） in patients with acute coronary syndrome（ACS） and discuss relationship between HSP60 and TNF-α. Methods： Ehzyme-linked immuhosorbent assay（ELISA） was used to detect the serum levels of HSP60 and TNF-α of 36 cases of stable angina pectoris（SAP） , 23 cases of ACS and 20 normal indivduals. Results： The level of serum HSP60 in ACS was significantly higher than those in control and SAP group（ P 〈 0. 001 ） , but there was no significant difference between SAP and control groups; For TNF-α, there was no significant difference among the three groups（ P 〉 0.05）. The serum HSP60 concentration had no positive correlation with TNF-α（ r = -0. 146,P 〉 0.05 ）. Conclusion： The level of serum HSP60 with ACS is elevated,which could reflect the status of immunity in ACS.