呼吸道合胞病毒蛋白G基因核苷酸分析

Nucleotide analysis of attachment protein G gene of respiratory syncytial virus

目的：对河南省呼吸道合胞病毒（RSV）进行分离与鉴定。方法：选择2006年冬季至2007年春季河南省部分医院急性呼吸道感染患者，采集咽拭子，利用Hep-2细胞和Vero细胞进行病毒分离，对分离株用特异性RT-PCR鉴定；对鉴定阳性的RSV毒株进行蛋白G全基因序列测定，并进行同源性分析，构建基因进化树。结果：共分离到4株RSV毒株，各分离株之间核苷酸同源性92％～99％；与A型RSV标准参考株的核苷酸同源性为86％～92％，与B型RSV标准株的同源性为70％。结论：2006年河南省RSV流行株为A型。

Aim： To isolate and identify respiratory syncytial virus（RSV） from Henan province. Method： RSV was isolated from swab collected from patients with syndrome of fever, cough in some Henan hospitals in the winter of 2006. The isolated strains identified by RT-PCR, were subjected to sequence the whole region of the G gene, and phylogenetic trees were constructed by comparison with RSV standard strains and reference strains. Results： Four RSV strains were isolated. The identities of the G＇gene among the four isolated strains were 92% -99% ,the identity of the G gene between the isolated strains and the reference A strain was 86% -92% ,but with the B strain, the identity was 70%. Conlusion：RSV prevalent in Henan province in the winter of 2006 is type A.