巴戟天醇提物对血淤大鼠血流动力学及血小板聚集的影响

Influences of Radix morinda officinalls ethanolic extracts on hemorheology and platelet aggregation in blood stasis rats

目的:观察巴戟天醇提物对血淤大鼠血流动力学及血小板聚集率的影响。方法:60只Wistar大鼠随机等分为正常对照组、模型组、复方丹参滴丸(0.1g/(kg.d))阳性药对照组及巴戟天醇提物12g/kg、6g/kg、3g/kg剂量组6组。正常对照组和模型组给予等体积的5g/L羧甲基纤维素钠溶液,其他各组每d1次灌胃给药,连续5d。采用2次皮下注射肾上腺素1mg/kg之间将大鼠置于冰水中5mim的方法建立大鼠急性"血淤"模型,观察药物对血淤模型大鼠高、中、低切变率下全血黏度、全血卡森黏度、血浆黏度、红细胞压积、全血还原黏度、红细胞电泳时间及二磷酸腺苷、胶原、凝血酶诱导血小板最大聚集率的影响。结果:巴戟天醇提物3个剂量组均能降低血淤大鼠的高、中、低切变率下全血黏度、全血卡森黏度,缩短红细胞电泳时间,抑制血小板最大聚集率(P均<0.01);其高、中剂量组尚能降低全血还原黏度(P<0.01或0.05)。结论:巴戟天醇提物可改善血淤时的血流动力学,抑制血小板聚集。

Aim： To investigate the influence of Radix morindae officinalls（RMO） ethanolic extracts on the hemorheology and platelet aggregation in acute stress blood stasis rats, Methods：A total of 60 Wistar rats were randomly separated into six groups （ 10 rats per group） ：normal control group, model group, Fu Fang Dan Sen Di Wan （0.1 g/kg） group, RMO ethanolie extracts 12 g/kg,6 g/kg,3 g/kg groups, Acute stress blood stasis rats model was prepared by leaving rats in ice water for 5 minutes between two times cutaneously injecting of hyodermic epinephrine 1 mg/kg,and the main hemorheological indexes and the rate of platelet aggregation induced by ADP, COL,and thrombin were detected, Three different concentrations （ 12 g/kg,6 g/kg,3 g/kg） of RMO ethanolic extracts was given daily for five days via oral administration, Results： 12 g/kg, 6 g/kg,3 g/kg treatment of RMO not only significantly reduced the increase of whole blood viscosity at either high, middle, or low shear rates, whole blood easson viscosity and red-cell electrophoresis time,but also restrained the rate of platelet aggregation （P 〈 0.01 ）, In addition, 12 g/kg,6 g/kg treatment of RMO ethanolic extracts also decreased the whole blood reduction viscosity in acute stress blood stasis rats （P 〈 0. 01 or 0.05）, Conclusion ： RMO ethanolie extracts has the ability of blood-activating and tasis-eliminating.