Q开关Nd:YAG激光非损伤嫩肤的小鼠动物实验研究

In vivo experiment study of non-ablative photorejuvenation using Q-Switched Nd:YAG laser in rats

目的:评估Q开关Nd:YAG激光非损伤嫩肤后小鼠真皮胶原蛋白含量的变化,为临床治疗提供理论依据。方法:采用532nm,1064nm的Q开关Nd:YAG激光;对32只小鼠随机分为4组,1组对照组,3组实验组。分别用532nm、1064nm以及这两种波长激光联合各照射5次,间隔7天。免疫组化技术检测32只小鼠真皮中Ⅰ和Ⅲ型胶原蛋白含量,HE染色测定真皮胶原纤维的厚度。结果:(1)胶原纤维的厚度及Ⅰ和Ⅲ型胶原蛋白的含量均比对照组高(P<0.01);(2)532nm组的胶原纤维的厚度及Ⅰ和Ⅲ型胶原蛋白的含量均比1064nm组高(P<0.01);两种波长联合的胶原纤维的厚度及Ⅰ和Ⅲ型胶原蛋白的含量均比532nm组和1064nm组高(P<0.01)。结论:532nm、1064nm以及这两种波长的激光联合照射是有效的嫩肤方法,532nm优于1064nm,两种波长联合优于单用一种波长。

Objective： To evaluate the change of the amount of collagen in the dermis after Q - Switched Nd： YAG laser non - ablative photorejuveantion, in order to offer reference for effective clinical treatment, nethods： Thirty- two rats were randomly divided into four groups including three laser irradiation groups and one control group. The rats＇ skin was irradiated by 532 nm, 1064 nm and a combination of 532 nm and 1064 nm Q- Switched Nd：YAG laser for five times at interval of seven days. The content of collagen type Ⅰand Ⅲ in the dermis was measured by immunohistochemical method. The thickness of collagen bundles in the dermis was measured under HE stain. Results： （1） The collagen fiber was thicker and the content of collagen type Ⅰand Ⅲ was higher in the experimental groups than in the control group （ P 〈 0.01 ）, in the 532 nm group than in the 1064 nm group （ P 〈 0.01 ）, and in the combination group of 532 nm and 1064 nm than in 532 nm alone and in 1064 nm alone （ P 〈 0.01 ）. Conclusion： A combined irradiation of 532 nm and 1064 nm yield better result than either used alone, and 532 nm irradiation is superior to 1064 nm.