70株志贺菌属血清分型及耐药分析

Serotyping and Susceptibility Test of 70 Shigella spp Strains

目的分析医院细菌性痢疾的流行菌株类型及耐药特点。方法对医院肠道门诊腹泻患者的粪便标本进行细菌分离培养、鉴定和血清分型,并以K-B琼脂法进行药敏实验。结果在检出的70株志贺菌属中,弗氏志贺菌37株、宋内志贺菌33株,未分离出志贺菌属的其他型别;70例菌株对传统抗菌药物中的四环素、复方新诺明、氨苄西林、利福平、萘啶酸的耐药率均>62%,对喹诺酮类的常用药诺氟沙星耐药率较低为12.8%,而头孢类的耐药率<10%。结论医院细菌性痢疾流行株主要是弗氏志贺菌和宋内志贺菌,多重耐药率较高,达到77.1%,临床治疗可首选头孢菌素类药物,庆大霉素和诺氟沙星次之。

OBJECTIVE To investigate the epidemic serotypes of Shigella and their antibiotic resistant characteristics in our hospital. METHODS The stool samples of the patients with diarrhea were collected and the bacteria isolation, culturing, and systemic serotype identification were made. At the same time, antibiotic sensitivity test was performed. The confirming test was made for the suspicious strains. RESULTS Of the 70 Shi- gella strains detected, 37 were group B （S. flexneri） and 33 group D （S. sonnei）. More than 62o/4 of the Shi- gella strains tested were resistant to tetracycline, SMZ/TMP, ampicillin, rifampicin, and nalidixic acid. But the resistant rate of Shigella strains to norfloxacin was 12.8%, and the resistant rate to cephalosporin was not higher than 10%. CONCLUSIONS In our hospital, the main serotypes of Shigella spp are S. flexneri and S. sonnei. Multidrug-resistant rate is 77. l%. Cephalosporin can be used in treating patients infected with Shigella as first choice,and secondly gentamicin and norfloxacin can be used.