卷烟接装纸、成型纸中荧光增白剂ABP、VBL的HPLC测定

Determination of Fluorescent Whitening Agents in Tipping Paper and Plug Wrapper with HPLC

采用高效液相色谱法同时检测了64个卷烟滤嘴成型纸、接装纸样品中的荧光增白剂ABP、VBL含量。即先用紫外灯照射纸样品，而后用N，N-二甲基甲酰胺提取显示荧光的样品，提取液经Alltima C18柱（150mm×4．6mm×5μm）色谱分离和甲醇水（体积比45：55，流速1．0mL／min）混合液洗脱，在波长435nm下检测。结果显示：①ABP、VBL的捡出限分别为0．10、0．15ng／mL，平均回收率101．0％～102．1％、102．0％-～04．2％，RSD 0．66％～2．48％、0．89％～1．84％；②60个纸样在紫外光下没有荧光；③4个有荧光的纸样中均未检出ABP，而都检出了VBL，其中，1个为接装纸样品，3个为成型纸样品。该法适用于批量卷烟滤嘴成型纸和接装纸中荧光增白剂ABP、VBL的同时测定。

The fluorescent whitening agents, ABP and VBL, in 64 samples of tipping paper and plug wrapper were determined with HPLC. The samples were irradiated by UV-lamp, and the samples which fluoresced were extracted with N, N - dimethylformamide. The resultant extract was passed through an Alltima C18 column （150 mm ×4.6 mm ×5 μm） using methanol-aqueous solution （45： 55, v/v） as mobile phase at a flow rate of 1. 0 mL/min, and detected at the wave length of 435 nm. The results showed that： 1 ） the average recoveries of ABP and VBL were 101.0% to 102.1% and 102.0% to 104. 2% with RSD of 0. 66% to 2.48% and 0. 89% to 1. 84% at detection limits of 0.10 and 0.15 ng/mL, respectively; 2） 60 samples did not fluoresce under UV- lamp ; 3） VBL was detected in the other 4 samples which fluoresced, while ABP was not ; among the 4 samples, 1 was tipping paper and 3 were plug wrapper. The method is suitable for the simultaneous determination of ABP and VBL in batches of plug wrapper and tipping paper samples.