咪唑克生对实验性自身免疫性脑脊髓炎的行为、病理和免疫的影响

Effect of Idazoxan on Behavior,Pathology and Immunology of Experimental Autoimmune Encephalomyelitis

目的:探讨咪唑克生(Ida)对实验性自身免疫性脑脊髓炎(EAE)的行为、病理和脊髓IL-12p40、IL-10、GFAPmRNA表达的影响。方法:用GPSCH免疫Wistar大鼠制作EAE模型,免疫后分别给予Ida0.5、1.5和4.5mg·kg-1。比较各组行为学和组织学的变化,用RT-PCR法测脊髓IL-12p40、IL-10和GFAP mRNA的表达。结果:与EAE组比Ida1.5mg·kg-1组和Ida4.5mg·kg-1组的行为学和病理变化减轻,脊髓IL-12p40mRNA表达降低,IL-10和GFAP mRNA表达升高(P<0.05)。结论:Ida对EAE有保护作用,可能与打乱促炎和抑炎细胞因子的平衡及促进星形胶质细胞增殖活化有关。

Aim： To investigate the effect of Idazoxan（Ida） on the behavior, pathology and expression of the mRNA of IL-12/23p40, IL-10 and glial fibrillary acidic protein（GFAP）,which is an intermediate filament protein in astrocye, and increases when it proliferates or is activated. Methods： EAE was induced by immunizing Wistar rats with guinea pig spinal cord homogenate. After immunization, rats were treated with Ida for ten days respectively at the dose of 0.5 mg·kg^-1,1.5 mg·kg^-1 or 4.5 mg·kg^-1, ip, bid. The ethologic and histopathologic change is observed, and the expression of the mRNA of IL-12/ 23p40, IL-10 and GFAP is detected by reverse transcriptase- polymerase chain reaction. Results ： In the Ida 1.5 mg·kg^-1 and Ida 4.5 mg·kg^-1 groups, the incidence decreased, the behavioral signs and inflammatory cell infiltration in central nerve system relieved. Spinal cords from rats of Ida 1.5 mg·kg^-1 and Ida 4.5 mg·kg^-1 groups showed decreased level of IL-12/23p40 mRNA and increased level of IL-10 and GFAP mRNA compared to EAE group. Conclusion： Idazoxan had protective effect on EAE. It is presumed that Idazoxan protects EAE by shifting the balance of pro-inflammatory and anti-inflammatory cytokines and promoting the activation of astrocyte.