摘要
目的分析生存素(Survivin)和血管内皮生长因子(VEGF)在原发性肝细胞癌(HCC)中的表达,探讨两者之间的关系。方法分别应用原位杂交法和免疫组织化学SP法检测HCC 50例、肝硬化30例、正常肝组织10例中Survivin mRNA、VEGF mRNA和Survivin蛋白、VEGF蛋白的表达情况。结果Survivin和VEGF在HCC、肝硬化和正常肝组织中的表达差异有统计学意义,Survivin在HCC中的表达高于肝硬化,而VEGF在肝硬化组织中的表达高于HCC中的表达。结论Survivin和VEGF在HCC中的表达关系密切,呈正相关;Survivin、VEGF的异常表达与HCC的发展密切相关,在HCC的发生、发展过程中起重要的作用。
Objective To explore the expressions of Survivin and VEGF and relationship between them in hepatocellular carcinoma(HCC). Methods The expressions of Survivin protein and VEGF protein in 50 HCC, 30 cirrhosis and 10 normal tissues were assessed by immunohistochemical method. The expressions of Survivin mRNA and VEGF mRNA in 50 HCC,30 cirrhosis and 10 normal tisst, es were assessed by in situ hybridization. Results The expressions of Survivin and VEGF in cancer tissues, cirrhosis tissues, normal tissues were significantly different. The expression of Survivin in HCC tissues was stronger than that in cirrhosis, but the expreesion of VEGF in cirrhosis was stronger than that in HCC tissues. Conclusion The expression of survivin is closely associated with the expression of VEGF in HCC and they take positive correlation. The abnormal expressions of Survivin and VEGF are closely associated with the development of HCC. They may play important roles in the development of HCC.
出处
《中国基层医药》
CAS
2007年第10期1647-1649,共3页
Chinese Journal of Primary Medicine and Pharmacy
关键词
生存素
血管内皮生长因子
肝肿瘤
免疫组织化学
Survivin
Vascular endothelial growth factor
Liver neoplasms
Immunohistochemistry