新疆传统发酵乳酪乳清对动脉粥样硬化大鼠血管内皮的保护作用

Effects of traditional fermented cheese whey on experimental atherosclerosis in rats

目的:观察新疆哈萨克族传统发酵乳酪的乳清(简称乳酪乳清)对实验性动脉粥样硬化大鼠内皮细胞损伤的保护作用。方法:通过灌胃高脂乳剂及注射大剂量维生素D_3致内皮损伤,建立动脉粥样硬化大鼠模型。高脂乳剂灌胃4周后给予乳酪乳清(25,50,100 mg·kg^(-1))及阳性对照药辛伐他汀(20 mg·kg^(-1)),给药4周后,分别测定血脂、肝脂、C-反应蛋白(CRP)、内皮素(ET)、血栓烷B_2(TXB_2)、肿瘤坏死因子(TNF-α)和白细胞介素-8(IL- 8)等指标。结果:乳酪乳清3个剂量组均显著降低动脉粥样硬化大鼠血清总胆固醇、低密度脂蛋白胆固醇和三酰甘油以及肝脏匀浆中的TG含量(均为P<0.01),均显著降低血清C反应蛋白水平(P<0.05),并降低血浆ET和血清TNF-α及IL-8水平(P<0.05)。结论:乳酪乳清具有调节血脂及保护内皮细胞作用,提示其抗实验性动脉粥样硬化作用与内皮保护作用有关。

Objective ： To determine the effect of the fermented cheese whey, a kind of traditional Kazakh medicine, on experimental atherosclerotic formation in rats. Methods： Sixty rats were treated with intragastric high-fat diet and injection of high-dose of vitamin D3 to induce injury of endothelial cells and atherosclerotic formation. From 4 weeks after above treatments, rats were randomly divided into 6 groups, and treated with fermented cheese whey （25, 50, 100 mg·kg^-1） or simvastatin （20 mg·kg^-1） for4 weeks. Then, plasma lipids, hepatic lipids, and serum C-reaction protein （CRP）, endothelin （ET）, TXB2, TNF-α and IL-8 were determined. Results： Compared with the atherosclerotic control, the total cholesterol, low density lipoprotein cholesterol and triglyceride in plasma, and IL-8, ET, TNF-α and CRP in serum were dose-dependently decreased in the fermented cheese whey groups. Conclusion： Fermented cheese whey, the traditional Kazakh medicine, has the antiatherogenic effect similar to simvastatin.