摘要
目的:合成聚乙二醇单甲醚接枝壳聚糖(monomethoxy poly(ethylene glycol)-grafted—chitosan,mPEG—g—CS),并制备自组装纳米球。方法:利用甲醛连接法将聚乙二醇单甲醚(monomethoxy poly(ethylene glycol),mPEG)接枝于壳聚糖(chitosan,CS)分子,得到聚乙二醇(poly(ethylene glycol),PEG)改性的壳聚糖衍生物,并通过傅立叶红外光谱仪(Fourier transform infrared spectroscopy,FT-1R),核磁共振仪(proton nuclear magnetic resonance,^1H—NMR)对产物进行结构表征;采用超声透析法制备自组装纳米球,并通过透射电镜(transmission electron microscopy,TEM),动态激光粒度分析仪(dynamic laser light scattering,DLLS)表征了纳米球的形态和粒径;以芘为荧光探针,通过荧光检测分析测定了mPEG—g—CS的临界胶束浓度(critical micellar concentration,CMC)。结果:通过FT—IR,^1H—NMR确证了接枝产物的存在;mPEG—g—CS在水溶液中能够自组装形成球状纳米胶束,平均粒径为250nm。结论:通过甲醛连接法制备mPEG—g—CS,具有制备方法简捷、反应周期短、易操作的优点。利用该产物制备的纳米球有望成为长循环纳米药物载体。
Objective :To synthesize methoxy poly( ethylene glycol)-grafted-chitosan and prepare its self-assembled nanoparticles. Methods : The monomethoxy poly ( ethylene glycol) -grafted-chitosan was synthesized by formaldehyde linking method. The chemical structure of polymer was characterized by the fourier transform infrared spectroscopy(FT-IR) and proton nuclear magnetic resonance (^1H-NMR). The self-assembled nanoparticles was prepared by probe sonication-dialysis method and analyzed by transmission electron microscopy (TEM), dynamic laser light scattering (DLLS). The critical micellar concentration(CMC) of monomethoxy poly(ethylene glycol)-grafted-chitosan was determined by measuring the fluorescence intensity of the pyrene as a fluorescent probe. Results:The chemical structure of polymer was confirmed by FT-IR and ^1H-NMR. The self-assembled nanoparticles were spherical and their average diameter was about 250 nm. Conclusion:The preparation procedure of monomethoxy poly(ethylene glycol)-grafted-chitosan was easy to be operated. This novel nanoparticle system was promising to be a good long-circulating drug carrier.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2007年第21期1780-1783,1787,共5页
Chinese Journal of New Drugs
基金
国家重大科学研究计划项目(2006CB933300)
博士点基金(96002323)
厦门市科技计划项目(3502Z20055007)
关键词
壳聚糖
聚乙二醇单甲醚
自组装
纳米
长循环
chitosan
monomethoxy poly ( ethylene glycol )
self-assembled
nanometer
long-circulating