蒙药嘎日迪-15中多糖的研究

Determination of Constituents of Polysaccharide and the Content of Saccharide in Mongolian Medicine Garidi-15

用热水提取蒙药嘎日迪-15中水溶性多糖,经SephadexC-25进行提纯精制得纯糖,采用硫酸-苯酚法测定了其水溶性多糖含量。方法的平均回收率为100.50%,RSD为0.82%。用GC测定了蒙药嘎日迪-15中水溶性多糖主要由木糖、甘露糖、半乳糖、葡萄糖组成,其摩尔比为1.03∶1.26∶0.77∶2.30。用溴化钾压片法测得的红外谱图显示多糖的特征吸收峰为3417.46,2928.65,1742.86,1643.69,1149.78,1078.19,1022.56,834.57cm-1,其中1078.19和1022.56cm-1为吡喃糖特征峰,834.57cm-1是α-吡喃糖苷键的特征吸收峰。紫外谱图在280nm处有明显的糖吸收峰,说明有CO键存在。

The present paper introuduces the method, with which the water-soluble polysaccharide was extracted with hot water from Garidi-15, the products were further purified with column chromatography on SephadexC-25, and the contents of water soluble polysaccharide were determined by phenyl hydrate-sulfuric acid method. The average recovery was 100. 50%, and RSD was 0. 82%. The components of water soluble polysaccharide were identified by gas chromatography as xylose, mannose, galactose and glucose in the molar ratio of 1.03 ： 1.26 ： 0. 77 ： 2. 30. The water soluble polysaccharide was identified by IR spectrum and UV（200-400 nm） scanning spectrum. The IR spectrum indicates that there are characteristic absorption peaks of polysaccharides at 3 417. 46, 2 928.65, 1 742. 86, 1 643.69, 1 149.78, 1 078. 19, 1 022.56 and 834. 57 cm^-1 , of which there are absorption peaks of pyranose at 1 078. 19 and 1 022.56 cm^-1, and contain one characteristic absorption peak of a-pyranose indican band at 834. 57 cm^-1. The UV scanning spectrum shows the obvious polysaccharide absorption peak at 280 nm, and indicates the existence of C-O bond.