骨再生修复材料的仿生制备及生物分子调控矿化

Biomimetic Preparation of the Composite for Bone Restoration and Mineralization Modulated by the Biomolecules

采用冷冻干燥和纳米合成技术,仿生制备出BG-COL-HYA-PS骨修复材料,并利用体外仿生矿化实验、生物组装技术、动物体内植入实验和组织形态学观察以及扫描电镜(SEM)、能谱微区分析(EDS)、X射线衍射(XRD)等技术,对材料分别在模拟体液、培养液中以及体内的生物矿化及其调控机制进行了研究.实验表明,胶原蛋白、透明质酸、磷酸丝氨酸等生物分子作为天然细胞外基质,在调控矿化方面起到了重要作用;所制备的材料可促进骨再生修复.

BG-COL-HYA-PS porous composite for bone restoration was prepared via the biomimetic method using refrigeration-desiccation and nano-synthesis techniques The mechanism of bio-mineralization and biomolecular modulation of the composite in the simulated body fluid, in the cell culture fluid and in vivo, were respectively investigated by means of the techniques such as biomimetic mineralization in vitro, bioassembly, implantation to animals, histological morphology observation, SEM/EDS and XRD. The results indicate that the natural extracellular matrixes such as collagen （COL）, hyaluronic acid （HYA） and phosphoserine （PS） play important roles in the modulation of mineralization. The prepared composite could effectively promote the new bone restoration.