离子交换色谱柱上橙皮苷分离与配位反应的一体化

Intergration of Separation and Coordination Reaction of Hesperidion in Ion-Exchange Chromatography Column

提出并实现了橙皮苷分离及其与Cu2+配位反应一体化的新设想,并借助离子交换色谱、紫外可见光谱、X-射线衍射、傅立叶红外光谱和核磁共振等分析手段探讨其作用机理.结果表明,在离子交换色谱柱上能有效实现配体分离与配位反应的一体化,制备高生物活性中药配合物新药.其反应机理在于树脂相中软碱橙皮苷与溶液相中交界酸Cu2+间存在库仑力,容易发生配位反应,配体的分子状态和疏水状态因Cu2+对橙皮素的"集中效应"和芸香二糖对橙皮素的"包埋效应"而改变,故再生剂阴离子易穿过能斯特层与橙皮苷交换,并因电子云重叠而排斥橙皮苷至液相中,从而有助于分离、反应一体化.

A novel technology about the integration of separation and coordination reaction of hesperidin with Cu^2＋ in ion-exchange chromatography （IEC） column was presented and performed in this paper. Ion-Exchange Chromatography, UV-vis, XRD FFIR and ^1 H-NMR were adopted to analyze the mechanism of the technology. The results show that it is feasible to produce new medicines with high biological activity by the column reaction between metal ions （Cu^2＋） and Chinese medicine, such as hesperidin. The reaction mechanism lies in the fact that as a soft base, anion hesperidin on resin can easily coordinate with Cu^2＋ insolution due to the electrostatic attraction between them, which modifies the molecular state and hydrophobic state of hesperidin by the ＂centralizing effect＂ of Cu^2 ＋ on hesperetin and the ＂embedding effect＂ of rutinose on hesperetin. As a result, the anions in the regenerant go through the Nernst film to replace hesperidin, and hesperidin disperses in the liquid phase due to the overlapping of electron clouds, thus contributing to the integration of separation and coordination reaction.