新疆经典型Kaposi肉瘤14-3-3β表达与细胞增殖、凋亡的关系

Relationship between 14-3-3β expression of classic type of Kaposi's sarcoma in Xinjiang and its proliferation and apoptosis

目的:探讨新疆经典型Kaposi肉瘤(Kaposi's sarcoma,KS)组织中14-3-3β蛋白表达与细胞增殖及细胞凋亡的关系。方法:应用免疫组织化学Envision二步法和原位细胞凋亡标记技术(TUNEL)对新疆10例经典型Kaposi肉瘤及对照组12例健康人正常皮肤组织进行14-3-3β表达、细胞增殖指数(proliferating index,PI)及细胞凋亡指数(apoptotic index,AI)的检测。结果:14-3-3β蛋白在KS组织中的阳性表达率显著高于在正常皮肤组织中的表达(P<0.001),其阳性反应程度在2组比较差异有统计学意义(t=14.661,P<0.01);KS组织中PI和AI均明显高于正常皮肤组织(t=5.427、2.712,P<0.05);KS组织中14-3-3β蛋白与PI、AI均呈正相关(r=0.770、0.879,P<0.01)。结论:14-3-3β的过表达与新疆经典型Kaposi肉瘤细胞的失控性增殖有关,KS细胞的凋亡与多种途径有关,并可能受到多种负向调节因子的作用。

Objective： To explore the relationship between 14-3-3β expression and proliferation and apoptosis of classic type of Kaposi＇s sarcoma （KS） in Xinjiang. Methods： Immunohistochemical Envision 2-step method was used to detect 14-3-3β expression in 10 cases of classic type of KS and 12 cases of normal skin tissues from healthy subjects. Cell proliferating index （PI） was measured. Cell apoptotic index （AI） was determined by the TdT-mediated dUTP nick end labeling （TUNEL） method. Results： The positive expression rate of 14-3-3β protein in KS tissues was significantly higher than that in normal skin tissues （ P 〈 0.001 ）. The degree of positive reaction was significantly different between KS and normal skin tissues （t = 14. 661, P 〈0.01 ）. The PI and AI of KS cells were significantly higher than that of normal skin tissues（ t =5. 427 and 2. 712 ,P 〈0.05 ）. The expression of 14-3-3β positively correlated with PI and AI （ r = 0.770 and 0. 879, P 〈 0.01 ）. Conclusion ： Over-expression of 14-3-3β is related with the loss of control of proliferation of classic type of Kaposi＇s sarcoma in Xinjiang. The apoptosis of KS is related with multiple pathways and regulates by several negative regulating factors.