RT-PCR法评价干扰素α-2b干粉吸入剂的药效学

Pharmacodynamic Evaluation of Interferon α-2b Inhalation Powder by RT-PCR

目的对重组人干扰素α-2b(IFNα-2b)干粉吸入剂的药效学进行评价。方法以IFNα-2b诱导的2′,5′-寡腺苷酸合成酶(OAS)为替代指标,建立了一种灵敏的、半定量的逆转录-聚合酶链反应(RT-PCR)法测定OAS两种亚型OAS1和OAS2在外周血单个核细胞中的mRNA水平。结果与市售注射剂相比较,自制IFNα-2b干粉吸入剂诱导的OAS1的mRNA水平相当,但OAS2的mRNA水平更高。结论重组人干扰素α-2b干粉吸入剂有望成为一种非侵害性的、依从性良好的、发挥全身性疗效的给药途径。

OBJECTIVE To evaluate the pharmacodynamic effects of recombinant human interferon α-2b （IFN α-2b） inhalation powder. METHODS 2′ ,5′-Oligoadenylate synthetase （OAS） was used as a surrogate marker for IFN α-2b induction, and a sensitive and semi-quantitative method was established by use of reverse transeription-polymerase chain reaction （ RT - PCR） for the determination of OAS1 and OAS2 forms of OAS mRNA in peripheral blood mononuclear cells. RESULTS OAS1 mRNA concentration was equivalent after IFN α-2h was administrated by injection and inhalation ,but the later administration route resulted in a higher OAS2 mRNA level. CONCLUSION IFN α-2b inhalation powder is promising to be a non-invasive systemic delivery route with excellent compliance.