福建病圃与大田稻瘟菌群体遗传谱系和毒性类型的比较

Composition of lineage and virulence type of the Magnaporthe grisea population derived from the nurseries and fields in Fujian Province of China

用rep-PCR方法分析了病圃和大田的稻瘟菌的遗传谱系组成,并在CO39NILs6个近等基因系品种上进行毒性类型分析。结果表明,不同群体之间的遗传谱系和毒性类型均不完全相同,福建稻瘟菌群体在年度间存在明显的优势谱系,1999、2000年的优势谱系均为CFL03,2001年为CFL07;两个季节中的谱系类型组成差异小,早季病圃和大田及晚季大田优势谱系均为CFL07。年度间病圃与大田的毒性类型组成和优势类型都有很大的变化,1999年病圃的优势毒性类型为I20.1,而2000年则为I24.1和I34.1两个类型;毒性类型I1.1、I5.1、I11.1、I26.1和I35.0只在1999年出现,而毒性类型I4.1和I14.1只在2000年出现。两个季节中病圃的毒性类型组成有所差异,早季有1个毒性类型(I35.1)在晚季未出现,晚季有4个毒性类型(I10.1、I31.1、I32.1、I21.1)在早季中未出现,优势毒性类型早季的I14.1变为晚季的I20.1,毒性类型组成也有很大差异,且晚季的比早季的丰富。

To better understand the population dynamics of the pathogens at disease nursery and field, we studied the pathogens population at two disease nurseries located in Shanghang and Jiangle counties and their nearby fields with virulence analysis based on CO39 near isogenic lines （CO39 NILs） and DNA fingerprint patterns defined by a repetitive element （Pot-2） based polymerase chain reaction （rep-PCR）. The prevalent lineage was CFL03 in 1999 and 2000, and was CFL07 in 2001. The prevalent lineage was CFL07 in early and late season in the nursery and field in Jiangle County. The prevalent pathotype was I20.1 in 1999 and were I4.1 and I14.1 in 2000. The prevalent pathotype was I14.1 in early season and was I20.1 in late season in 2001. The use of traditional and molecular techniques will improve the efficiency of screening new varieties for resistance. It will also be beneficial to deepen the understanding of the relationship between the pathotype and genetic lineage, and to breed for durable resistance in rice varieties.