山羊Callipyge基因型的检测

Detection of Goat Callipyge Genotype

本试验扩增了波尔山羊、南江黄羊和承德无角山羊3个品种113个个体的Callipyge基因长度为493 bp的片段。经测序,发现一个SNP位点,第184 bp处C→T。对Callipyge限制性酶切位点(Fok I)的PCR-RFLP的多态性进行分析,在波尔山羊和南江黄羊两个品种中表现多态性,得到CC、CT和TT 3种基因型,波尔山羊群体中基因型频率分别为0.6571、0.3143、0.0286,南江黄羊群体中基因型频率分别为0.8947、0.1053、0.0000,而承德无角山羊全部为CC基因型。研究结果提示184C→T可能与山羊双肌性状有关。

The 493bp fragment of Callipyge gene of 113 individuals was amplified by PCR, of which 35 were from Boer goat, 38 were from Nanjiang brown goat and 40 were from Chengde polled goat. There was a variation of C184T discovered by sequencing. Molecular genetic diversity of Callipyge gene with restriction endomuclease Fok I was studied by PCR-RFLP. The polymorphism was existed in Boer goat and Nanjiang brown goat. There were 3 genotypes, which was CC, CT and TT, respectively. The frequency of genotypes was 0. 6571, 0. 3143 and 0. 0286 respectively in Boer goat. The frequency of genotypes was 0. 8947, 0. 1053 and 0. 0000 respectively in Nanjiang brown goat. However, there is all CC genotype in Nanjiang brown goat. It suggested that there was a relationship of the variation of C184T to the trait of Callipyge in goat.