黑冠鹤性别的分子鉴定方法

The Molecular Sexing of Balearica Pavonina

黑冠鹤,也称戴冕鹤,在我国数量较少,对其进行人工繁殖与人工育种意义很大。本研究采用非伤害性采样方法,利用黑冠鹤羽毛提取基因组DNA,之后利用2个引物组合对黑冠鹤EE0.6基因上的相关片段进行了特异性扩增。结果显示,这2个引物组合都能从雄鹤中扩增出1条片段,从雌鹤中扩增出2条片段,从而成功地对黑冠鹤的性别作出了准确判断,有利于黑冠鹤的成功配对和繁殖。

Balearica pavonina has a small quantity and it＇s significant to develop artificial reproduction and breeding. This research extracted genome DNA from feathers and amplified the EE0.6 gene of Balearica pavonina specifically with two combinations of primers. The results showed that male birds displayed one distinct amplification band,whereas two bands were amplified for female ones. With the results, the sex of Balearica pavonina could be identified successfully. It is meaningful for the reproduction of the birds.