奶牛体外受精胚胎的不同培养方法对比试验

IVF Embryo of Cow Different Culture Method Contrast Experiment

以屠宰场奶牛卵巢为试验材料,研究体外、体内培养法对体外受精胚胎发育的影响。①体外受精胚胎分别在加输卵管上皮细胞单层和颗粒细胞单层(2×106个/ml)的体外培养体系的培养液中培养与临时受体绵羊体内培养对比试验。结果显示:在培养的第8 d囊胚发育率无显著差异,体外培养体系囊胚形成主要集中在第8 d(受精日为第0 d)。体内培养体系囊胚的形成多数在第7 d。加体细胞的体外培养体系与体内培养法囊胚生成率无显著差异,但却显著好于不加体细胞的简单体外培养法。②进行2种方法生产的胚胎的程序冷冻、解冻敏感性试验。结果显示:体内培养法和普通体外法生产的胚胎解冻后存活率(90%;60%)和囊胚孵化率(85.7%;44.4%)存在极显著差异(P<0.01)。

As experiments the material take the slaughter house cow ovary, studies in vitro, in vivo cultivates the method of preserving health to the external fertilization embryonic development influence. （1） IVF embryo in vitro culture system whether adds the oviduct epithelial cell monolayer （granular cell monolayer）（2 × 10^6/ml） in nutrient fluid culture and temporary acceptor sheep in vivo raise contrast experiment. Result demonstration that, In the culture 8 d BL embryo growth rate not remarkable difference, in vitro culture system BL embryo forms mainly concentrates in 8 d（fertilization date for 0 d）. In vivo culture system BL embryo＇s formation most in 7 d. Adds the somatic cell in vitro culture system and in vivo cultivates the method of preserving health BL embryo production rate not remarkable difference, but remarkable is actually better than not add the somatic cell simple in vitro to cultivate the method. （2）two methods to produce the embryo the procedure to freeze, the thawing sensitivity test. Result demonstration that, After in vivo cultivates the method of preserving in vivo and the ordinary in vitro law production embryo defrosts, the survival percentage （90% VS 60%） and the embryo hatching rate （85.7% VS 44.4%） have the remarkable difference （P〈0. 05）.