摘要
以制备的多抗血清为基础,建立了氧氟沙星(OFL)、二氟沙星(DIF)和诺氟沙星(NOR)的多残留间接竞争酶免疫吸附测定法(Ci-ELISA法)。OFL、DIF和NOR的标准曲线的线性回归方程分别为y=-0.1984x+0.9687(r=0.9885)、y=-0.1738x+0.9150(r=0.9985)、y=-0.2004x+0.9572(r=0.9954);中值(IC50)分别为231.57、245.321、86.14 ng/ml;最低检测限(LOD)分别为1.62、1.861、.07 ng/ml;标准曲线在2~10000 ng/ml之间均有良好的线性关系(r≥0.9885)。方法的批内CV≤5.44%,批间CV≤12.45%。当OFL、DIF或NOR以浓度15~10000 ng/ml在牛奶中添加时,药物能较好地被回收,回收率均在71.70%~94.53%之间。
The antiserum was prepared with the mixture of OFL-BSA,DIF-BSA and NOR-BSA. The Ci-ELISA method for the detection of ofloxacin ,difloxacin and norfloxacin was established with it. To ofloxacin , difloxacin and norfloxacin. The regression equations about their standard curves were y=-0. 1984x+0. 9687(r=0. 9885),y=-0. 1738x+0. 9150 (r=0. 9985) and y=- 0. 2004x+ 0. 9572 (r= 0. 9954), respectively. The 50% inhibition concentration(IC50 ) were 231. 57,245. 32 and 186.14ng/ml, respectively. The limits of detect (LOD) were 1.62,1.86 and 1.07ng/ml , respectively. The coefficients of variation for the Ci-ELISA method were Less than 5.44 % in intra-assay and 12.45% in inter-assay. The recoveries in milk ranged from 71.70% to 94.53%.
出处
《中国畜牧兽医》
CAS
2007年第11期126-129,共4页
China Animal Husbandry & Veterinary Medicine