氟喹诺酮类药物的多残留酶免疫分析研究

Study on the Multi Residues of Fluoroquinolones by Enzyme Immunoassay

以制备的多抗血清为基础,建立了氧氟沙星（OFL）、二氟沙星（DIF）和诺氟沙星（NOR）的多残留间接竞争酶免疫吸附测定法（Ci-ELISA法）。OFL、DIF和NOR的标准曲线的线性回归方程分别为y=-0.1984x＋0.9687（r=0.9885）、y=-0.1738x＋0.9150（r=0.9985）、y=-0.2004x＋0.9572（r=0.9954）;中值（IC50）分别为231.57、245.321、86.14 ng/ml;最低检测限（LOD）分别为1.62、1.861、.07 ng/ml;标准曲线在2～10000 ng/ml之间均有良好的线性关系（r≥0.9885）。方法的批内CV≤5.44%,批间CV≤12.45%。当OFL、DIF或NOR以浓度15～10000 ng/ml在牛奶中添加时,药物能较好地被回收,回收率均在71.70%～94.53%之间。

The antiserum was prepared with the mixture of OFL-BSA,DIF-BSA and NOR-BSA. The Ci-ELISA method for the detection of ofloxacin ,difloxacin and norfloxacin was established with it. To ofloxacin , difloxacin and norfloxacin. The regression equations about their standard curves were y=-0. 1984x＋0. 9687（r=0. 9885）,y=-0. 1738x＋0. 9150 （r=0. 9985） and y=- 0. 2004x＋ 0. 9572 （r= 0. 9954）, respectively. The 50% inhibition concentration（IC50 ） were 231. 57,245. 32 and 186.14ng/ml, respectively. The limits of detect （LOD） were 1.62,1.86 and 1.07ng/ml , respectively. The coefficients of variation for the Ci-ELISA method were Less than 5.44 % in intra-assay and 12.45% in inter-assay. The recoveries in milk ranged from 71.70% to 94.53%.