聚酰胺分离纯化柑桔皮中总黄酮的工艺研究

研究了聚酰胺分离纯化柑桔皮中总黄酮工艺的影响因素,确立了聚酰胺树脂分离纯化桔皮总黄酮的最佳工艺,即上样浓度1.5mg/mL,上柱流速为3BV·h-1,再以3BV体积分数为80%的乙醇解吸附,流速3BV·h-1。桔皮黄酮经上述工艺3次纯化后,总黄酮含量达到45.26%。

The purification technology of total flavonoids from orange peel with polyamide was studied, with the content and recovery rate of flavonoids as indexes. The result showed that the optimum conditions of purification were as follows： the loaded concentration of flavonoids from orange peel was 1.5 mg/mL, flow velocity was 3BV·h^-1, the desorption agent was 80% ethanol of 3BV at 3BV·h^-1 . The content of flavonoids was 45.26% after purification with polyamide for three times.