人参皂苷对Aβ诱导单核细胞培养上清液致神经细胞损伤的影响

Effects of Ginsenoside on Nerve Cells Injuried by Aβ-Induced Monocyte Supernatant

目的研究人参皂苷对β-淀粉样肽(Aβ)诱导THP-1单核细胞培养上清液致SK-N-SH神经细胞损伤模型的影响及其作用机制。方法用乳酸脱氢酶(LDH)漏出率观察SK-N-SH细胞的损伤程度。用放射免疫法测定THP-1单核细胞培养上清液中炎性细胞因子IL-1β,IL-8和TNFα的浓度。结果人参皂苷与THP-1单核细胞预孵育30 min,再用Aβ1-40(125 nmol.L-1)与THP-1细胞孵育2 h,离心取上清液加入到SK-N-SH神经细胞培养中孵育24 h。结果显示,GS(100和200 mg.L-1)可明显减少LDH从神经细胞漏出,减轻细胞膜损伤;人参皂苷(50,100和200 mg.L-1)显著减少THP-1单核细胞培养上清液中细胞炎性细胞因子IL-1β,IL-8和TNFα的含量。结论人参皂苷可以通过抑制炎性细胞因子的表达而减少对神经元的损伤,因此对于AD的防治具有潜在的应用价值。

OBJECTIVE To investigate the effect and mechanism of ginsenoside on nerve cell damage model caused by the supernatant of THP-1 monocytes induced by ,8-amyloid （Aβ）. METHODS The cellular impairment was determined by LDH leakage from human neuroblastoma cell line SK-N-SH cells. The concentrations of i^fflammato^- cytokine IL-1β, IL-8 and TNFα were measured by radioimmunoassay （RIA） in the supernatant of THP-1 monocvtes. RESULTS THP-1 monocytes were preincubated with ginsenoside for 30 rain, then incubated with Aβ1-40 （ 125 nmol · L^-1） for 2 h; after centrifuge, their supernatant was added to SK-N-SH cell culture and incubated for 24 h. The results showed that GS （ 100 and 200 mg· L^-1 ） significantly decreased LDH leakage from SK-N-SH nerve cells, ginsenoside（50, 100 and 200 mg · L^-1 ） obviously reduced the contents of inflammatory, cytoklne IL-1β, IL-8 and TNFα in the supernatant of THP-1 monocytes. CONCLUSION Ginsenoside can protect nerve cells from damage by inhibiting the expression of infflammatory cytokines in monocytes induced by Aβ, thus may have the potential for treating Alzheimer disease.