ICP-MS同时分析中药材中7种微量元素的方法研究

Determination of 7 Trace Elements in Traditional Chinese Medicine by ICP-MS

目的建立用电感耦合等离子体质谱（ICP—MS）同时分析中药材中铜（cu）、砷（As）、镉（Cd）、汞（Hg）、铅（Pb）、硒（Se）、锗（Ge）7种元素的方法。方法样品经微波消解后，以钪（^45Sc）、钇（^89Y）、铟（^115In）、铋（^209Bi）作为内标物质，直接用ICP-Ms分析上述7种元素，并通过标准曲线计算含量。结果7种元素标准曲线的线性关系良好，相关系数（r）在0．9993～0．9999之间；检出限0．019～0．107μg·L^-1；加样回收率88．92％～115．0％；5次平行实验的分析结果在3d内稳定性良好，且所有元素的相对标准偏差（RSD）均小于10％；除砷（As）略低外，标准物质茶叶（GBW07605）中其他元素的测定值都在标准值范围内。结论该方法是一个灵敏、可靠、稳定的中药材多元素分析方法。

OBJECTIVE To establish a method for simultaneous analysis of 7 elements （Cu, As, Cd, Hg, Pb, Se, Ge） in Traditional Chinese Medicines （TCM） by Inductively Coupled Plasma Mass Spectrometry （ICP-MS）. METHODS With ^45 Sc, ^89Y, ^115 In and ^209Bi as internal standard elements, the sample solutions were analyzed by ICP-MS after microwave digestion, and the contents of elements were calculated according to their calibration curves. RESULTS The correlative coefficients of calibration curve for all ele- ments were higher than 0. 099 3 and the limits of detection were between 0. 019μg · L^-1 and 0. 107μg · L^-1. The analytical values of parallel tests of samples （ n = 5 ） were stable in three days and the relative standard deviations for all elements were lower than 10%. The average spiked recoveries ranged from 88.92% to 115.0% and the analytical results of tea SRM （ GBW 07605 ） showed acceptable agreement with the certified values. CONCLUSION This method is a sensitive , reliable and stable method for the multi - elements determination of Traditional Chinese Medicine by ICP-MS.