液相色谱-电喷雾离子阱质谱联用确证小鼠灌胃给药CTN986后血与脑组织中的药物原型

Determination of the parent drug of CTN986 in mice blood and brain after ig administration by LC-ESI-ion-trap-tandem MS

目的:确定 CTN986小鼠灌胃给药后血与中枢神经系统(CNS)中的药物原型。方法:血浆、脑组织样品用甲醇沉淀蛋白,离心后取上清液经固相萃取后用液相色谱-离子阱质谱进行测定。结果:小鼠灌胃给药后的血和脑组织样品经液质测定,所得的选择离子流色谱图(m/z 741)及一、二、三级质谱图均与对照品 CTN986选择离子流色谱图、各级质谱图一致。结论:小鼠灌胃给药后,CTN986能以原型的形式吸收入血并且可以通过血脑屏障进入中枢。

Objective ： To identify parent drug of CTN986 in mice blood and brain after ig administration. Methods： Samples of plasma and brain homogenate were added methanol to precipitate proteins and supernatants were identified by high performance liquid chromatography - electrospray ion trap mass spectrometer after solid phase extraction. Results： The SIM （m/z 741 ）chromatograph and bar graphs of MS1 , MS2 and MS3 of plasma and brain homogenate samples conform to their respective standard. Conclusion：After mice were administered via ig, CTN986 was absorbed into blood and distributed to brain through the blood - brain barrier in the form of parent drug.