HPLC法同时测定肾通颗粒中6种成分的含量

Simultaneous HPLC determination of six components in Shentong granules

目的:建立同时测定肾通颗粒的6种成分—淫羊藿苷、补骨脂素、异补骨脂素、淫羊藿次苷Ⅱ、隐丹参酮及丹参酮Ⅱ_A 含量的分析方法。方法:色谱柱:Alltech C_(18)(4.6 mm×250 mm,5μm);流动相:水(A)-乙腈(B),梯度洗脱法;检测波长:270nm,245 nm;柱温:30℃;流速:1.0mL·min^(-1);进样量为20μL。结果:淫羊藿苷、补骨脂素、异补骨脂素、淫羊藿次苷Ⅱ、隐丹参酮及丹参酮Ⅱ_A 的线性范围分别为0.30～2.97,0.05～0.50,0.03～0.32,0.12～1.16,0.06～0.56,0.10～1.00μg范围内,线性关系良好(r>0.9995)。平均加样回收率(n=6)分别为100.7%,99.0%,101.2%,101.8%,98.0%,100.1%;RSD<2.0%。结论:本方法操作简单、重复性好,为评价和控制肾通颗粒的质量提供了可靠的方法。

Objective：To develop a method for determining the six components--icariin, psoralen, isopsoralen, icarisid- Ⅱ, cryptotanshione, tanshione ⅡA in Shentong granules simultaneously. Methods： The analytical column was Alhech C ts （4. 6 mm × 250 mm,5 μm）. The mobile phase was water （A） -acetonitrile （B） with gradient elution. The detection wavelengths were 270 nm and 245 nm. The flow rate was 1.0 mL·min^-1 and the column temperature was 30 ℃. The sample injection volumn was 20 μL. Results：Good linearities of icariin, psoralen, isopsoralen, icarisid- Ⅱ , cryptotanshione, tanshione ⅡA were showed within the ranges of 0. 30 - 2.97,0. 05 - 0.50,0.03 - 0. 32,0. 12 - 1.16,0. 06 - 0. 56,0. 10 - 1.00 μg respectively, r 〉 0. 9995. The average recoveries （ n = 6 ） were 100. 7%, 99.0% ,101.2% ,101.8% ,98.0%, 100. 1% respectively, RSD 〈 2. 1%. Conclusion：The method is simple and show a good reappearance, which can be used for the quality control of this preparation.