高效液相色谱法测定痹痛消膏中虎杖苷的含量

HPLC determination of polydatin in Bitongxiao ointment

目的:建立痹痛消膏中虎杖苷的含量测定方法。方法:采用高效液相色谱法,色谱柱为 Phenomenex Luna C_(18)柱(250 mm×4.6 mm,5μm),乙腈-水(21:79)为流动相,流速1 mL·min^(-1),检测波长:306 nm。结果:虎杖苷在0.016～0.32μg范围内呈良好的线性关系,r=0.9999。精密度、重复性、稳定性均良好,RSD 分别为1.6%,1.7%,1.1%;平均加样回收率为101.1%(n=9)。结论:所建立的方法稳定、可靠,可为该产品建立质量控制方法提供参考。

Objective：To establish a method for determination of polydatin in Bitongxiao ointment. Methods：A Phenomenex Luna C 18 column （ 250 mm× 4. 6 mm, 5 μm） was adopted with CH3 CN - H2O （ 21： 79） as mobile phase. The flow rate was 1 mL· min^-1 ,detection wavelength at 306 nm. Results：The linearity range was 0. 016 -0. 32 μg, r = 0. 9999. The average recovery rate was about 101.1% （n =9）. Conclusion ：This method is accurate and reproducible, and availed for quality control of the Bitongxiao ointment.