荧光分光光度法直接测定阿曲库铵

Determination of atracurium by fluorescence spectrophotometry

目的:建立简便快速的直接测定阿曲库铵含量的荧光分光光度法。方法:应用荧光分光光度法测定阿曲库铵的含量,激发波长为280 nm,发射波长为317 nm。结果:在0.088～7.5μg·mL^(-1)范围内阿曲库铵浓度和荧光强度呈线性关系。检出限(3σ)为26.5 ng·mL^(-1)。考察了体系的光谱特征、适宜的反应条件和共存物质的影响。结论:方法具有良好的选择性,可用于人体血清、尿样及合成样品中阿曲库铵的测定。

Objectives： To develop a fluorescence spectrophotometry method for determination of atracurium. Methods： A fluorescence spectrophotometry method was applied to the determination of atracurium. The excitation wavelength was located at 280 nm. The emission wavelength was located at 317 nm. Results： The calibration curves was linear between intensity of fluorescence signals and the concentration of atracurium in the range of 0. 088 -7. 5 μg ·mL^-1. The detection limit（3σ）was 26. 5 ng · mL^-1. The spectra characteristics ,the optimum reaction conditions and influencing factors had been discussed. Conclusion： The method is simple, rapid, direct and selective. It has been applied to the determination of atracurium in human serum,urine and synthetic samples with satisfying results