HPLC法测定栽培河套大黄中土大黄苷的含量

HPLC determination of rhaponticin in Rheum hotaoense C.Y.Cheng et C.T.Kao

目的:建立 HPLC 法测定栽培河套大黄中土大黄苷的含量。方法:采用 ZORBAX Eclipse XDB—C_(18)(150 mm×4.6 mm,5μm)色谱柱,以甲醇-乙腈-水(35:5:60)为流动相,流速:1.0 mL·min^(-1),检测波长为320 nm,采用外标法定量测定。结果:土大黄苷进样量在0.206～2.06μg范围内与峰面积呈良好的线性关系(r=0.9998);平均回收率(n=6)为99.31%,RSD 为0.82%。结论:本方法操作简便,结果准确可靠,适用于栽培河套大黄中土大黄苷的含量。

Objective：To establish an HPLC method for the content determination of rhaponticin in Rheum ho- taoense C. Y. Cheng et C. T. Kao. Method： A ZORBAX Eclipse XDB - Cis column （ 250 mm × 4. 6 mm, 5 μm） was used as the analytical column and mobile phase was methanol -acetonitrile -water（35： 5： 60）at a flow rate of 1.0 mL·min^-1 and the UV detection wavelength was set at 320 nm. Results： The linear range was 0. 206 - 2. 06μg（ r = 0. 9998 ） for 1 rhaponticin ;The average recovery （ n = 6 ） of rhaponticin were 99.31%, RSD = 0. 82%. Conclusion： This method is simple, rapid and reliable for determination of rhaponticin in Rheum hotaoense.