基质金属蛋白酶-3基因启动子5A/6A多态性与高血压病的关系

Association between the matrix metalloproteinase 3 promoter 5A/6A polymorphism and essential hypertension

目的探讨基质金属蛋白酶-3(MMP-3)基因启动子-1171(5A/6A)多态性对兰州地区汉族人群高血压病发病的可能影响。方法采用限制性片段长度多态性分析技术,检测高血压病患者(高血压病组,104例)和正常者(对照组,102例)MMP-3基因启动子SNP-1171(5A/6A)基因型的分布。结果高血压病组与对照组MMP-3基因型分布差异无统计学意义,按性别分组比较两组男性、女性之间差异均无统计学意义(P<0.05),Logistic回归分析发现男性50岁以下病例组与对照组比较差异有统计学意义(P=0.013)。并且患高血压的危险性将增加5.97倍。不同血压分级之间基因型分布和等位基因频率比较无差异(分别为P=0.950,P=0.885)。结论MMP-3基因启动子-1171(5A/6A)多态性对高血压病发病的影响与性别年龄有关,与血压水平无关。

Objective to investigate the possible impact of the polymorphism in 5A/6A MMP-3 gene promoter on the risk of essential hypertension in Han Lanzhou area population. Methods 104 hypertensive patients（ hypertensive group） and 102 normal persons control were recruited. Polymerase chainreaction followed by restrictionfragment length polymorphism （PCR-RFLP） was used to detect SNP-1171 （5A/6A） in MMP-3 gene promoter region. Results The distribution of MMP-3 genotype was not significantly different between hypertensive patients and controls. After dividing Study sample by gender, Distribution of MMP-3 genotype and allele frequencies also were not significantly different between hypertensive and normotensive neither males nor females （ P 〈 0.05 ）. When Multiple logistic regression analysis was used, we found 5.97-fold increased risk for hypertension associated with genotype 6A/6A （P = 0.013 ） in young males （ 〈 50 years） according to recessive model. No significantly differences among different blood pressure level. Conclusion These results indicate that there was gender and age-specific differences in association of MMP-3 5A/6A polymorphism with hypertension in Lanzhou Han population. No marked association could be found between 5A/6A polymorphism in MMP-3 gene and different blood pressure level （ P =0. 950.P =0. 885 ） .