可溶性白细胞分化抗原14和可溶性肿瘤坏死因子受体-P55治疗严重烫伤小鼠实验研究

Experimental Studies on the Therapic Effect of sCD14 and sTNFR-P55 on the Serious Scalded Mice

目的:观察可溶性白细胞分化抗原14(sCD14)和可溶性肿瘤坏死因子受体-P55(sTNFR-P55)对严重烫伤模型小鼠炎症反应、胰岛素抵抗和胰岛素分泌的治疗效果。方法:模型小鼠单独及联合静脉注射sCD14和sTNFR-P55,观察不同治疗组以及治疗对照组(模型组)、正常对照组的死亡率、外周血白细胞(WBC)总数、稳态模式胰岛素抵抗(HOMA-IR)指数和胰岛素分泌(HOMA-β)指数以及空腹血糖和乳酸含量变化。结果:sCD14和sTNFR-P55联合组小鼠死亡率明显低于治疗对照组(P<0.05);联合组及单一治疗组WBC总数、HOMA-IR指数、空腹血糖和乳酸含量均明显低于治疗对照组,且联合组低于单一治疗组;但HOMA-β指数治疗组明显高于治疗对照组(P<0.01),且联合组高于单一治疗组(P<0.01)。结论:静脉注射sCD14和sTNFR-P55能够有效改善创伤炎症反应、胰岛素抵抗和胰岛β细胞功能障碍,降低创伤模型小鼠病死率,且联合治疗效果优于单一治疗。

OBJECTIVE： To study the therapic effect of soluble cluster of differentiation （sCD14） and soluble tumor necrosis factor receptor-P55（sTNFR-P55） on the inflammation,insulin resisitance and insulin secretion of serious scalded mice. METHODS： The lecels of mortality, peripheral white blood cell（WBC）, Homeostasis Assessment-Insulin Resistance （HOMA-IR） and Homeostasis Assessmen- β（HOMA-β）, fasting glucose, fasting lactic acid of the scalded mice treated individually by infusion with sCD14,sTNFR- P55,and both the sCD14 and sTNFR-P55, were examined compared with that of the control. RESULTS： The mortality of the scalded mice treated both by infusion with both of sCD14 and sTNFR-p55, were lower than that of the control（P 〈 0.05）. The levels of WBC, HOMA - IR, HOMA- β, fasting glucose, fasting lactic acid of treated scalded mice were all lower than that of the control scalded mice（P〈0.01）, and that of the scalded mice individually treated by sCD14 or sTNFR- p55 were lower than that of both treated by sCD14 and sTNFR-P55（P〈0.01）. CONCLUSIONS： The treatment with sCD14 and sTNFR- P55 by mainline could contribute to ameliorate the inflammation, insulin resistance, disfunction of islet β cell, reduce the traumatic mortality. The therapic effect of treatment with both the sCD14 and sTNFR - P55 was better than that of treatment with single one.